Spider Crabs Lab Report

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Researchers at the Alfred- Wegener- Institute for Polar and Marine Research in Bremerhaven, Germany have recently published a peer-reviewed article about the effects of temperature and ocean acidification on the calcium content of young spider crab larvae, Hyas araneus. Kathleen Walther, Franz J. Sartoris, and Hans O. Pörtner decided to research these effects to determine if the continued temperature and acidification of the oceans will have a negative effect on the spider crabs development. Furthermore, the researchers measured the calcium content in each larvae of crab at different temperatures and CO2 content. For the spider crabs, the calcium levels they have are crucial for development of their exoskeleton. Lower levels of calcium in the crab could be detrimental to the development of it because of …show more content…

In each temperature increment there were three different flasks filled with different levels of CO2 in the water to show the effects of ocean acidification on the larvae. The first flask was filled with present day CO2 levels of 380 parts per million (ppm), the second was filled with 710 ppm CO2, and the third was filled with 3,000 ppm CO2. The last two flasks are estimated of what the ocean CO2 levels will be in year 2100 and 2300, respectively. The results of each flask were assessed on days 3, 7, and 14 and were tested to find out the levels of calcium the larvae contained. However, not all larvae were tested because the researchers used random sampling to test the larvae to because this created separation from the few mothers of each larvae. To test the calcium content of the larvae the researchers used ion chromatography. Ion chromatography separates different ions to show the content of each ion in the larvae, so this shows the calcium levels because calcium ion can be separated to show the

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