Finding the Concentration of a Sample of Sulphuric Acid The purpose of our experiment is to find the concentration of a sample of sulphuric acid accurately. We are provided with a range of indicators, solid anhydrous sodium carbonate and access to all laboratory glassware and equipment. Apparatus needed: - ------------------- Requirements to produce a Sodium Carbonate solution: - ====================================================== Electronic Balance (accurate to 2 decimal places) Goggles Spatula Distilled Water (250cm3) Anhydrous Sodium Carbonate (2.65g) Beaker - 250cm3 Stirring Rod Petri Dish Pipette Volumetric flask Requirements for the titration experiment: - ============================================ Goggles Burette (50cm3) Stand and Clamp Conical flask Sulphuric (IV) Acid (n*50cm3 based on how many repeats are conducted) Prepared Sodium Carbonate Solution Methyl Orange Indicator (10cm3) Funnel Pipette Pipette filler White Tile Method ------ Method for the production of Sodium Carbonate solution ====================================================== 1. Use the volumetric flask to get 250cm3 of distilled water 2. Place 2.65g of sodium carbonate in a petri dish (weighed on the electronic balance).
(SDS denatures the proteins, BPB is used as a tracking dye while Beta Mercaptoethanol breaks sulfide bonds.) Heat the samples at 80 degrees Celsius for 5 minutes in a water bath. This helps in protein denaturation. Put the cassette into the buffer dam and fill it with the running buffer. For SDS-PAGE, running buffer is prepared by taking 40 ml Tris-Glycine and adding 4 ml of 10% SDS to it.
Now add ~ 0.35 g of zinc powder to the solution and stir until the solution becomes clear. Dissolve the excess zinc with more sulfuric acid. Decant the liquid with a stirring rod, retaining only the copper. Rinse the copper with distilled water and steam dry. Weigh the mass.
Repeat this process for a total of 10 teaspoons. Second Test In a 100ml beaker place 50mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved.
Standardising: 1.) Rinse burette with distilled water and then with a little of the dilute HCl solution. 2.) Drain burette into your waste beaker and fill it with acid. 3.)
5ml of starch was put into a test tube. The test tube was placed into the beaker. When the water in the beaker was at the required temperature the stop clock was started. After one minute 1ml of amylase was put into the test tube with the starch using a syringe or pipette. As soon as the amylase and the starch had mixed a sample was taken from the test tube using a pipette and mixed with the droplets of iodine in one of the chambers of the spotting tile.
Repeat for each trial. Rinse volumetric pipette with vinegar and drain into the waste beaker. Weigh and record the mass of each 200mL beaker. Add 10.00mL of vinegar into each beaker and weigh them and record their again. Add 50mL of de-ionized water to the beakers and place them under the drop counter on top of a stir plate, submerging the pH meter into the solution.
· Measure out 20cm3 of sodium thiosulphate using a measuring cylinder and pour it into a conical flask. · Place the conical flask on top of the white paper with the cross on it and put both the paper and the conical flask on the bench mat. · Clean the measuring cylinder out with distilled water. · Measure out 20cm3 of 0.1M HCI with the measuring cylinder. · Add all the 20cm3 of HCI into the flask · Start the stop clock place the flask on the piece of paper, when it goes cloudy stop the clock and record time · Tidy up.
Secondly I will set out the apparatus and get all the equipment from the list (shown on the previous page). Using the measuring cylinder, I will put 20cm3 of distilled water into a boiling tube, and take the starting temperature. In order to make this a fair test I will burn the fuel for one minute only, use 20cm3 of water and make sure the tip of the fuel’s flame is under the boiling tube. After the minute is up, I will blow out the flame or place the lid of the fuel over it, (cutting off the oxygen). I will then take the finial temperature.
How the Concentration of Sodium Thiosulphate Influences Speed of Reaction with Dilute Hydrochloric Acid Aim: I am investigating how the concentration of sodium thiosulphate influences the speed of reaction with dilute hydrochloric acid. Equipment ========= * 1 piece of paper with a cross on it * 1 timer * 1 conical flask 100ml * 1 test tube brush * 119ml Sodium thiosulphate * 14ml 100% Hydrochloric acid * 1 beaker 400ml * 1 beaker 10ml 1. Working with the first dilution I will measure out the solution and place the Sodium thiosulphate in the conical flask positioned on top of the paper so the cross is visable from above, through the neck of the flask. Then I will measure out and add the water needed in that dilution. 2.