Sa-Mcf In Cattle

Describe the pathogenesis of SA-MCF in cattle. Sheep-associated malignant catarrhal fever (SA-MCF) is a pathogenetic virus that affects several types of animals including cattle. There are several characteristics signs of this infection including a high mortality rate but low morbidity rate in the infected population. Research has suggested that the incubation time for MCF is between two and ten weeks; however, one study showed that the incubation time could last up to nine months. In a recent outbreak at a state fair, it took an average of 71 days for MCF to kill the cattle. The virus is generally transmitted from sheep to cattle; it seems to be transmitted to animals in captivity at a higher rate and has a more devastating effect due to the close proximity. MFC is a fatal

The OvHV-2 specific antibodies prevented infection in these cells. However, when the cells were exposed to the negative serum, neutralization was not detected. The results of this experiment led the researchers to conclude that neutralization assays for this virus can give insight into the presence and number of neutralizing antibodies for a heterologous protein. Replacing the AIHV-1 gB gene by OVHV-2 homolog had no effect on the capability of AIHV-2 to invade and infect other mammalian cells. In addition, the AIHV-1ΔORF73/OvHV-2-ORF8 caused the formation of antibodies against the OvHV-2 gB protein which meant the virus was not pathogenic in rabbits. The data obtained from these results suggest that the AIHV-1/OvHV-2 virus could potentially be used as a vaccine for SA-MCF. Since anti OvHV-2 antibodies can neutralize the AIHV-1ΔORF73/OvHV-2-ORF8, this could be used to recognize and quantify the OvHV-2 gB neutralizing antibodies in vitro.

Why were rabbits used in this study? Describe the pathogenesis of OvHV-2 and AIHV-1 in