Methods
To determine the rates of photosynthesis for the 5-week-old bean plant and the 5-week-old corn plant, the QUBIT Systems, Inc. apparatus was employed. A diagram of this apparatus is presented in page 4-5 of the biology lab manual (Bio, 2002). The computer connected to this apparatus was adjusted to present two graphs which measure the percentage of Oxygen in the air over time and photon flux (micromole quanta/m^2/s). Next, a leaf was retrieved from the top of a bean plant and sealed from the air in the laboratory via thumbscrews inside the leaf chamber of the apparatus. Caution was used to ensure that the leaf was not placed directly beneath the oxygen sensor of the apparatus, since doing so would result in improper amount of surface area of the leaf being exposed to sunlight. To ensure that the leaf was not over heated by the lamp's light, a petri dish was filled with water and placed directly over the leaf. Next, the mouse of the computer with the Logger Pro software was used to start the plot of the two graphs: %Oxygen / Time (s) and photon flux (micromole quanta/m^2/s) / Time (s) (maximum time for plot was 30 minutes). The students then filled a bag with their breath and connected it to the leaf chamber to flush out the laboratory air. The chamber was resealed, the lamp was adjusted to maximum luminance, and the graph was observed until the amount of oxygen being produced reached equilibrium, at which point the plot was stopped and printed off. The graph of %oxygen over time was analyzed for its slope (Beta sub-1) by the logger pro software in the stage at the beginning where oxygen production was slow, the stage where it was linear, and the stage where it was decreasing to equilibrium. The leaf was then removed from the chamber and the surface area of the leaf within the chamber was determined using a transparent grid. This procedure was then repeated in the exact same steps with a 5 week old corn plant leaf. The photosynthetic rate for each of the three stages and each leaf was then determined using the following formulas.
Rate of Oxygen Production (Bio,2002):
(Micromoles of Oxygen/L/min (T=Temperature=25C))
Rate of Oxygen Production as a function of leaf surface area (Bio, 2002):
Photosynthetic Rate=(Micromoles of Oxygen/m^2/min)
Results
The collection of data of the slope of each leave+s' oxygen production yielded two charts below, one entitled Bean and one Corn.
Cellular respiration is the process by which energy is harvested involving the oxidation of organic compounds to extract energy from chemical bonds (Raven & Johnson, 2014). There are two types of cellular respiration which include anaerobic respiration, which can be done without oxygen, and aerobic respiration, which requires oxygen. The purpose of this experiment is to determine whether Phaseolus lunatus, also known as dormant seeds or lima beans, respire. You will compare the results of the respiration rate of the dormant seeds, and the Pisum sativum, or garden peas. In this experiment, you will use two constants which will be the temperature of the water and the time each set of peas are soaked and recorded. Using these constants will help
In this experiment I will investigate the affect in which the light intensity will have on a plants photosynthesis process. This will be done by measuring the bubbles of oxygen and having a bulb for the light intensity variable.
Investigating the Effect of Light Intensity on the Size of a Plantain Leaf Title: To investigate the effect of light intensity on the size of a plantain leaf. Hypothesis: I predict that the size of the plantain leaves would increase as the light intensity decreases. Therefore, plantain leaves found in the shade will have larger surface areas than leaves found in an open area. Theory: Sunlight is an essential factor need to complete the process of photosynthesis.
The Effect of Light on the Organic Plant Elodea Aim: To calculate the rate of photosynthesis from the number of oxygen bubbles produced by the plant. Photosynthesis: The process by which green plants use the sun's energy to build up carbohydrate reserves. Plants make their own organic food such as starch. Plants need Carbon dioxide, water, light and chlorophyll in order to make food; and starch and oxygen are produced. Carbon dioxide and water are the raw materials of photosynthesis.
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
The Effect of Light Intensity on the Rate of Oxygen Production in a Plant While Photosynthesis is Taking Place
= > [CH2O} + O2 + H2O, This shows that when the light intensity is increased the rate of reaction will be more quicker he only anomalous result there was, is the one in the 100 watt result the reading after 5 minutes is anomalous because it does not follow the predicted pattern of increasing in the production of gas because it is lower I know from my own knowledge of photosynthesise that when the light intensity is increased the rate of reaction will be more quicker because many plants and trees photosynthesise quicker in stronger light and photosynthesise slower in dimly lit places. The chlorophyll absorbs light energy and enables it to be used by the plant for building up sugar. The overall effect is that energy is transferred from sunlight to sugar molecules.
Comparing the Growth of Pea Plants Grown in the Light and in the Dark Aim: To compare the vertical growth and weight gain of pea plants grown in the light and in the dark. Background Knowledge: Photosynthesis forms the basis for this experiment. This is the process by which a plant makes food for itself from the raw materials around it. The energy needed for photosynthesis comes from sunlight, which is the variable for this experiment.
The Effect of Light Intensity on Photosynthesis Of Elodea Canadensis Introduction I wanted to find out how much the light intensity affected the Photosynthesis in Elodea Camadensa. I decided to do this by measuring the amount of oxygen created during photosynthesis. Photosynthesis is the procedure all plants go through to make food. This process uses Carbon dioxide, water and light energy. It produces Oxygen and Glucose.
Two members of the group were instructed to visit the laboratory each day of the experiment to water and measure the plants (Handout 1). The measurements that were preformed were to be precise and accurate by the group by organizing a standardized way to measure the plants. The plants were measured from the level of the soil, which was flat throughout all the cups, to the tip of the apical meristems. The leaves were not considered. The watering of the plants took place nearly everyday, except for the times the lab was closed. Respective of cup label, the appropriate drop of solution was added to the plant, at the very tip of the apical meristems.
* Count the number of bubbles seen in 1 minute which is a way of
The “Fast Plant” experiment is an observation of a plants growth over the span of twenty-eight days. The objective is to observe how plants grow and use their resources throughout the span of their life. In our lab we observed the Brassica rapa, a herbaceous plant in the mustard family which has a short cycle which makes it a perfect plant to observe in this experiment. Like other plants the Brassica rapa must use the resources in the environment to create energy to complete itʻs life cycle and reproduce. By observing the plant it is easy to see in what organ or function the plant is using itʻs energy and resources and if overtime the resources switch to other part of the plants. By conducting this experiment we are able to observe where and how plants allocate their resources throughout their life by harvesting plants at different points in their life.
Investigating the Effect of Light Intensity on Photosynthesis in a Pondweed Aim: To investigate how the rate of photosynthesis changes at different light intensities, with a pondweed. Prediction: I predict that the oxygen bubbles will decrease when the lamp is further away from the measuring cylinder, because light intensity is a factor of photosynthesis. The plant may stop photosynthesising when the pondweed is at the furthest distance from the lamp (8cm). Without light, the plant will stop the photosynthesising process, because, light is a limited factor. However once a particular light intensity is reached the rate of photosynthesis stays constant, even if the light intensity is the greatest.
The Effect of Light Intensity on the Rate of Photosynthesis in an Aquatic Plant Introduction The input variable I will be investigating is light, as light is just one of the 4 factors required in the green-plant process of photosynthesis. Photosynthesis is the process by which green-plants use sunlight, carbon dioxide, water & chlorophyll to produce their own food source. This process is also affected by the temperature surrounding the plant (the species of plant we experimented with, pond weed, photosynthesised best at around 20 degrees centigrade.) Light, temperature & CO2 are known as limiting factors, and each is as important as the next in photosynthesis. Light is the factor that is linked with chlorophyll, a green pigment stored in chloroplasts found in the palisade cells, in the upper layer of leaves.
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.