Introduction The purpose of this lab was to determine the size of prokaryotic microbes in comparison to eukaryotic cells. Students used oil immersion microscopy to magnify the microbes and compare their size to that of a eukaryotic cheek cell. The results of this lab allowed students to compare and contrast eukaryotic and prokaryotic cell size, which is a necessary skill when working with microbiology based labs. Microbiology is the study of microscopic organisms, also known as microbes. Microorganisms include bacteria, fungi, protists, and autotrophic organisms.
Most useful to the fields of biochemistry and molecular genetics is the use of these methods in gene identification. First a gene must be isolated from an organism. This can be accomplished using restriction enzymes, cutting the DNA into pieces and then inserting these pieces into plasmid vectors, creating a library of genes. These vectors are then inserted into bacteria, which proceed in replicating the genes and producing their products. Any bacteria producing the protein of interest are isolated, using radiolabeled antibodies which bind specifically to the target protein.
I am writing this research paper on the differences and similarities between bacteria, viruses, and prions. In this paper I will define each of the subjects that I am comparing and tell and show you how they are different and how they are similar. I will also tell you about their structure, how they reproduce, and a disease that is caused by each of them. I will first start with defining all three subjects. Bacteria are known as large groups of unicellular microorganism that have cell walls but lack organelles, a nucleus, and bacteria are also prokaryotic.
We will also examine the diversity of prokaryotic cell types. For example some are pathogenic, causing diseases, while other are saprophytic, breaking down organic material, and further still some bacteria are autotrophs, making their own food through photosynthesis or chemiosmosis (Lab Manual, 2001). We will also examine the characteristics of these prokaryotic organisms such as the visible size, form, color, elevation, and texture of a colony or colonies. To get detailed observations of these various organisms we will view their occurrence through the culturing of samples by adding microbes onto a sterile medium within Petri dishes and allowing them to multiply. Finally we will also examine the features of cyanobacteria that distinguish them from other bacteria, and their role in nature.
There are many different cells that do many different things. But all of these cells fall into two categories: prokaryotic and eukaryotic cells. Eukaryotic cells contain a nucleus and are larger in size than prokaryotic cells. Prokaryotic cells do not contain a nucleus, are smaller and simpler than eukaryotic cells. Two of their similarities are they both have DNA as their genetic material and are covered by a cell membrane.
The main goal of this experiment was to determine if DNA or proteins within bacteriophages were the hereditary material that entered a bacterial cell to direct the assembly of new viruses. This experiment followed in its predecessor’s footsteps and used tools developed in the field of microbiology for the observation of cellular behavior. The researchers split their main objective into two experiments. In one experiment researchers took a bacteriophage encoded with the element P, which is prominent in DNA but not proteins, and in the other experiment researchers used the bacteriophages(phages) holding the element S, which exists in proteins but not DNA. The mixtures were then allowed to infect bacteria.
AIM: The final goal for performing this project is to find out the crystal structure, active binding sites and functional analysis of the domains present in the HldE protein. By finding these important factors of the protein, and making the changes in active sites can result in altering the lipopolysaccharide (LPS) layer formation in bacteria. This helps antibiotics to act more prominently towards the specific kind of problems occur in those bacterial kinds. To achieve this, we need to perform the sequential comparison of the protein with other structurally existing proteins. Along with this, we need to purify the protein without any tags for forming crystals.
Step by step instructions for this procedure can be found in Benson’s, Microbiological Applications p. 99. Furthermore, an aseptic technique must be performed for this test and the entire tests following the unknown. The purpose of this test is to differentiate between gram positive and gram-negative bacteria. The key indicator of gram-positive bacteria is a purple stain and a pink stain for gram-negative bacteria. A slide is viewed with a microscope under oil immersion.
Microbiology is the study of microscopic organisms such as virus, Protista and bacteria. It’s important to know and to identify what kind of bacteria and how we can treat it since is found everywhere in society. Also to know what kind of bacteria it is by the performing different biochemical test and be able to differentiate the bacteria. This is use in the medial field where is important to know what kind of bacteria there dealing with and know how to treat it. The purpose of the study was to identify what are unknown bacteria by applying all the methods that we have learn in microbiology for the identification of are unknown.
Introduction The gram stain experiment is used mostly to help identify bacteria inside certain products. Also, the gram stain was being used since 1884 to separate bacteria into groups. The gram stain is separated based on the reaction on the stain. Bacteria reacts based on being tested with gram positive, gram negative and gram variable. Also, based from the scientist knowledge the response that occurs from cells to the stain occurs, because of the difference in the complexity and chemistry of the bacterial cell wall.