Phd Personal Statement

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The university's Ph.D. program is an exceptional and powerful program that will provide me with the opportunity to broaden my skills by virtue of additional experience. In effect, it will further prepare me for a brighter future in biochemistry research. My desire to continue my education via pursuit of a Ph.D. is so that I can become the independent scientist that I ought to be and accordingly, so that I can be a leader in the research and development process. I am an excellent candidate for the university's Ph.D. program considering that I am a motivated individual with ample prior research experience.

Throughout the past few years as an undergraduate, I have gained extensive exposure to biochemical research. The captivating nature of …show more content…

I started off in Dr. Jared Anderson's bioanalytical chemistry laboratory with the research topics regarding the use of magnetic ionic liquids (MILs) as solvents for rapid DNA extraction and preconcentration from aqueous samples for further downstream experiments. Most notably, I played a key role in the development of several methods for the subsequent recovery of DNA from the nucleic acid-enriched MIL microdroplet, including the development a solid phase extraction chromatography technique in addition to a method allowing for the incorporation of a biological sample-enriched MIL droplet into PCR for amplification of the targeted gene. Additionally, I conducted biophysical chemistry research under the direction of Dr. Michael Harris at Case Western Reserve University, where I studied the directional processing of polycistronic valVW and valWV ptRNA constructs by E. coli RNase P. Overall, it was concluded that fast cleavage occurs at both the valW and valV sites, rather than in a 3' to 5' directional ordered processing. Currently, I am working in Dr. Donald Ronning's biochemistry lab where I am studying Mycobacterium tuberculosis citrate synthase II structure to function relationships and carboxy terminus CXX post-translational fatty acid modifications in mycobacterium. Particularly, I obtained and refined a 2.1 Å crystal structure of citrate synthase containing the desired electron density for incubated pyruvate. Furthermore, I am working to test the hypothesis that the reactive thiol functional group at the −3 position of a protein is undergoing fatty acid modification in mycobacteria, a common occurrence among eukaryotes for protein translocation to the plasma

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