INTRODUCTION At what pH levels does the Periplaneta americinegar with pH of 2.9, milk of magnesia with pHh of 10.5 ,tomato juice with a ph of 4.1, clorox with a ph of 13 and milk with ph of 6.6.ana die in? In this experiment we will find out what pH levels are deadly for the Periplaneta Americana by exposing them to different pH levels. The Periplaneta Americana will be exposed to vinegar with pH of 2.9, milk of magnesia with pH of 10.5 ,tomato juice with a pH of 4.1, clorox with a pH of 13 and milk with pH of 6.6. HYPOTHESIS If the Periplaneta Americana are exposed to a pH level lower than 5 then all of the Periplaneta Americana will die. VARIABLES IV: the vinegar with pH of 2.9, milk of magnesia with pH of 10.5 ,tomato juice with a …show more content…
Experimental group: Periplaneta Americana exposed to vinegar, clorox, juice, milk and milk of magnesia. Control group: the Periplaneta Americana that were killed by the spray insect killer with a pH level of constants: the place where the substance will be put, the number of Periplaneta Americana in each trial. The size of each Periplaneta Americana needs to be the same. The amount of substance that is applied to the Periplaneta Americana . …show more content…
Pour 25 ml of vinegar in one of the 30 ml beakers. put 10 Periplaneta americana in a 10 oz plastic cup. Grab a pipette and add 20 drops onto the Periplaneta americana, you will put 2 drops on each periplaneta americana wait for 2 minutes, then record on a table how many Periplaneta Americana are dead in the plastic cup. put 10 new Periplaneta Americana in a new plastic cup. Pour 25 ml of clorox in one of the 30 ml beakers. Again grab a pipet and add 5 drops onto each of the Periplaneta americana . Wait for 2 minutes, then record on a table how many Periplaneta americana are dead in the plastic cup. Put again 10 new Periplaneta americana into a new plastic cup. Now pour 25 ml of milk in one of the 30 ml beakers. Once again grab a pipette and add 5 drops onto each of the Periplaneta americana . Wait for 2 minutes, then record on a table how many Periplaneta americana are dead in the plastic cup. Put again 10 new Periplaneta americana into a new plastic cup. Finally pour 25 ml of milk of magnesia in one of the 30 ml beakers. Grab a pipette and add 5 drops onto each of the Periplaneta
Each subsequent trial will use one gram more. 2.Put baking soda into reaction vessel. 3.Measure 40 mL vinegar. 4.Completely fill 1000 mL graduated cylinder with water.
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
To begin the lab, the variable treatment was prepared as the Loggerlite probe, used to later measure oxygen consumption, warmed up for approximately 10 minutes. To prepare the variable treatment, 200ml of Sodium and Ammo-lock water was measured in a container and a pre-prepared “tea bag” of tobacco was steeped in the room temperature treated water until a light yellow color was visible. After preparing the tobacco solution the preparation for the live goldfish began as two beakers were filled with 100 ml of treated water. Each beaker was weighed before addi...
After the addition of the media, we insert an aeration tube inside and cover the lid with a cotton plug and start giving them aeration. This preparation has to be put on for 3 days under proper sunlight and 25-30 degree Celsius to observe if the culture is healthy/ potent or not depending on the color each culture portrays (The nanochloropsis culture should have a grass-green color to be seen as potent and the isochrysis culture should have a dark brown color to be seen as potent), if the colors seem dull and light, then that might mean that the culture is impotent.
For this lab investigation, our question was do pillbugs prefer vinegar or plain water in their environment? Our hypothesis was that if they were given the choice, then they would choose the water side because their natural environment is not as acidic as vinegar. After we tested this hypothesis, the data that we collected over the course of 20 minutes supported our theory. At the end of the first minute, there were 3 pillbugs on the vinegar side, and 37 pillbugs on the water side. Then, after 10 minutes, there were 4 pillbugs on the vinegar side, 30 pillbugs on the water side and 6 pillbugs that were missing. After 15 minutes, 4 pillbugs were on the vinegar side, 28 were on the water side and 8 were missing. Finally, at the end of the 20th minute, there were 3 pillbugs on
Put the test tubes of Sodium Thiosuphate into the beaker above the Bunsen burner 3. Put a thermometer into it 4. Remove the test tube once its reached the desired temperature and pour contence into the empty beaker 5. As you begin to pour the test tube of Hydrochloric Acid contence into the beaker start the stop clock 6. When the cross on the paper becomes obscured stop the clock and record the result 7.
Attach a buret clamp (located under the hood) to a ring stand. b. Rinse the burets three times with approximately 10 ml of deionized water. Tilt and rotate the buret in an almost horizontal position (don't let the water spillout!) to rinse the entire inside wall. Allow about 5 ml of water to run through the buret tip on the last rinse. c. Pre-rinse one buret with approximately 5 ml of your Unknown acid solution. Again, rotate the buret to rinse the entire inside wall of the buret as above. d. Clamp the buret in one side of the buret clamp. Place a white piece of paper labeled "Unknown acid" under this buret. Drain any remaining pre-rinse acid solution into a beaker labeled "waste solution". e. Fill this buret with your Unknown acid solution to the zero mark or slightly below it (Not above the zero mark). Make sure the tip of the buret is completely filled and contains no air bubbles. f. Pre-rinse the second buret with approximately 5 ml of standard base solution. Clamp the buret in the other side of the buret clamp. Place a white piece of paper labeled "Standard NaOH solution" under the buret. Drain remaining prerinse NaOH solution into the waste solution beaker. Fill this buret with standard
Repeat for each trial. Rinse volumetric pipette with vinegar and drain into the waste beaker. Weigh and record the mass of each 200mL beaker. Add 10.00mL of vinegar into each beaker and weigh them and record their again. Add 50mL of de-ionized water to the beakers and place them under the drop counter on top of a stir plate, submerging the pH meter into the solution. Place the stir bar into the beaker and carefully turn on the stir plate so that the stir bar spins without splashing or hitting the sides of the beaker or the pH
The EDTA tube required 0.5 M EDTA to be added until the solution reached 1.25 mL of EDTA. To the Control tube, distilled water had to be added. Once done, this tube now contained 1.25 mL of water. After these steps were done, each tube called for three drops of milk. The tubes must then be inverted and allowed to sit for one minute.
Use glassware as directed by your instructor. Place a test tube placed inside a beaker with ice water to collect the product from the apparatus. Obtain the 10mL round bottom flask from the apparatus. Obtain two graduated cylinders of 10mL. On one graduated cylinder measure 4mL (85% H3PO4) of Phosphoric Acid and pour into the 10mL round bottom flask. On the other graduated cylinder measure 3mL of Cyclohexanol and pour into the flask as well. With a pipet add 5 drops of Sulfuric Acid (H2SO4) into the flask. Attach the round bottom flask to the distillation apparatus. Place thermometer with rubber stopper on the apparatus to obtain the temperature Start with the water flow through the condenser. Turn on and heat the reaction until the product starts to distill. Distill and collect until thermometer temperature rises to 85˚C. Once there is no more product to collect obtain the test tube of product. Two layers should be formed, top layer of cyclohexane and bottom layer with water. Obtain a pipette and remove the bottom layer (water) if any. Add 10% (5mL) of Sodium Bicarbonate (NaHCO3) to nuclearize any acid in the solution. Mix well and remove once again the bottom layer of water with pipette. Add 5mL of water and mix well to wash the top layer. After the two layers form again, remove entirely the bottom layer of water and add a few pellets of Calcium Chloride. Obtain a 50mL or 100mL beaker and weigh.
Rinse a 25mL buret with three 5mL portions of standard permanganate solution. Fill the buret with the standard permanganate solution and record initial and final readings.