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Colorimetry experiment lab report
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Task 4 Report outlining the function of; Microscope A microscope is an instrument which allows you to see things that the human eye cannot see nakedly. The simple function of a microscope is to magnify the specimen which is placed under it. Some microscopes are so strong that they can allow us to see things such as cells inside leaves and the internal structures of them. Balance The function of a balance is to measure the weight of a substance. They come in different accuracies and sensitivities. Spring scales measure weight by balancing the force of gravity against the force on the spring inside the scales, whereas a balance or pair of scales using a balance beam compares masses by balancing the weight due to the mass of an object against the weight of a known mass or masses. A digital spring balance will be much more accurate than a balance beam. PH meter A PH …show more content…
It consists of a graduated glass tube with a stopcock (a tap) at one end. Colorimeter A colorimeter is a device used in colorimetry, it refers to a device which measures the absorbance of particular wavelengths of light in a solution, and gives us a numerical value. Multimeter A multimeter is an electronic measuring instrument which holds the ability to measure several functions all in one small handheld unit. A standard multimeter can usually measure; voltage, current, and resistance. Practical carried out Instruments used Reasons why these instruments were chosen Serial dilutions Colorimeter Graduated pipette We used a colorimeter so that instead of getting an answer which told us whether the substance was absorbent or not. Were gained a result telling us how absorbent the substance was with an accurate numerical value. We used a graduated pipette as this instrument accurately measures small volumes of liquid, which is what we were dealing
Furthermore, using a graduated cylinder with markings below the 100 mL line would have allowed for more accurate measurements of the initial volume of air in the graduated cylinder.
Compress the safety bulb, hold it firmly against the end of the pipette. Then release the bulb and allow it to draw the liquid into the pipette.
The absorbance of these mixtures is measured at a suitable wavelength. If 'x' mole/litre are added to (1-x) mole/litre of M and if C1, C2
The color that was chose to be shined through the sample was purple. The spectrophotometer was set at a wavelength of 400nm to represent the purple color. It was zeroed using the blank meaning the spectrophotometer read zero as absorbance amount. The blank consisted of 5mL of water and 2.5 mL AVM and it was placed in cuvette. A solution with a known concentration of 2.0x10-4 M was used in the spectrometer. For this solution, 5 mL of the solution with 2.5 mL of AMV was placed in the cuvette. The cuvette was placed inside of spectrophotometer and the amount of absorbance was recorded. This procedure that involves a solution with a known concentration was repeated for the concentrations:1.0x10-4 M,5.0x10-5 M,2.0x10-5M, and1.0x10-5M.A unknown solution absorbance was measured by putting 5 mL of unknown solution with 2.5 mL AMV in a cuvette. The cuvette was placed in the spectrophotometer and the amount of absorbance was recorded. The procedure that deals with the unknown solution was repeated 2 more times with the same solution and the same amount of solution and AMV. The average of the three unknown solution was calculated and the concentration of the unknown solution was
I have to pull two alleles (two straws) from the bag to represent one fish because fishes like humans get two alleles one from their father and one from their mother.
Because it is a way of knowing the pressure that the blood is putting on the walls of arteries and veins.
Okay, if our lithium weight is going to be 6.941 g/moL Then that means we have to take 24.6g of Lithium and multiply it by 1 mol of Lithium over 6.941 g of Lithium. This would equal to be 3.544 mol of Lithium. Then we have to take that 3.544 and multiply it by 1 mol of hydrogen gas over 2 mol of lithium. Which would then equal into 1.772 mol of hydrogen gas. We can then figure out that 1.772 is our “n”. The “T” is our 301 Kelvin, the “P” is our 1.01 atm and the “R” is our 0.0820 which would be the L atm over mol k. And we can’t forget about our “V” which would be V equals nRT over P which equals 1.772 mol divided by 0.0820 L atm over mol kelvin multiplied by 301 kelvin over 1.01 atm which equals to our final answer of: 43.33 of H2
In the process of this experiment, there were a total of two bilingual aphasics and eight monolingual aphasics who were tested through nine different EF test batteries to measure their level of EF which includes behavioural inhibition (response inhibition & interference control), working memory, planning/problem solving and reconstitution. The nine EF test batteries consists of the Stroop Color Word Test, Trail Making Test, Self-Ordered Pointing Test, Complex Figures, Wisconsin Card Sorting Test, Tower of London, Raven’s Progressive Matrices, Five Point Test and Design Fluency. The main focus of the experimentation was to test these 10 different individuals through conversation to investigate their EF profiles. To attain these results, each
It is an attack by our best friends, …… and these attacks on mostly in randomly generated user name sites it was easy to short.
... samples before the incubation of 108 seconds. Then the 100 µL of colour reagent was put to the sample, merged and incubated for further 10 minutes. The absorbance at 615nm and 700nm wavelengths was calculated on the samples in the Cobas analyser and the sample concentration was measure according to :
A low absorbency would have a low color change so would be clear or slightly clear by the end of the trails and a high absorbency would have a strong red color by the end of the experiment.
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
The Standards, their concentrations and their absorbance are listed in the table below the graph. The x-axis is the concentration of the Bovine Serum Albumin, while the y-axis is the absorbance reading that was received when the spectrometer was set to 595nm.
In this lab, solutions were separated by polarity and affinity to solids by chromatography. Chromatography is the separation of a mixture, where the components move at different rates up a medium. The medium used was chromatography paper, matched with a series of developers to aid in movement of compounds upwards. The distance moved up the paper is measured and the rf is calculated. The distance the pigments traveled is divided by the distance developer traveled. The more polar a substance the further it travels up the paper. The paper works by capillary action and absorption to separate the compounds.
A plastic pipette was cut so that it was shorter than the length of the test tube. The test tube was put in with the stem down, and that so the bulb was loosely sealed off the tube.