Notes On Catalase

Results Cuvettes #2-7 and cuvettes #9 and #11 measured every 20 seconds all increased under the time constraints of 120 seconds. Cuvette #2 contained 1 mL of enzyme Catalase, measured at 23°C or room temperature and a pH of 8 had a rate of enzymatic reaction of 0.0013 absorbance/second. Cuvette #3 contained 2mL of enzyme Catalase, measured at 23°C and a pH of 8 had a rate of enzymatic reaction of 0.0028 absorbance/second. Cuvette #4 contained 4mL of enzyme Catalase, measured at 23°C and a pH of 8 had a rate of enzymatic reaction of 0.0041 absorbance/second. (Figure 1) Cuvette #5 contained 1mL of enzyme Catalase, measured at 0°C or freezing point and a pH of 8 had a rate of enzymatic reaction of 0.0037 absorbance/second. Cuvette #6 contained 1mL of enzyme Catalase, measured at 36°C and a pH of 8 had a rate of enzymatic reaction of 0.0029 absorbance/second. Cuvette #7 contained 1 mL of enzyme Catalase, measured at 100°C or boiling point and a pH of 8 had a rate of enzymatic reaction of 0.0015 absorbance/second. (Figure 2)

Cuvette #9 contained 1mL of enzyme Catalase, measured at 23°C, and a pH of 7 had a rate of enzymatic reaction of 0.0006 absorbance/second. Cuvette #11

The rate of enzymatic reaction was 0.0037 absorbance/second. Cuvette #6 contained 1mL of enzyme Catalase, measured at 36°C or warm water and a basic pH of 8. The rate of enzymatic reaction was 0.0029 absorbance/second. Cuvette #7 contained 1 mL of enzyme Catalase, measured at 100°C or boiling point and a basic pH of 8. The rate of enzymatic reaction was 0.0015 absorbance/second. Cuvettes 2, 5, 6, and 7 were all tested for the effect of temperature on enzymatic reactions. All of the enzymatic rates decreased as the temperatures of the solutions increased. The only flux between the enzymatic rates were from cuvette #2 and cuvette #5 because cuvette #2 was the control/baseline of the