Morphological Changes to Vero Cells Infected with Rubella
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Background: Estimation of the size of the cultured cells can be a valuable parameter. The present research was conducted to evaluate the modifications in the volume and surface area of the Vero cells using the invariator, nucleator, and surfactor techniques. Morphologic changes of the cells infected with rubella virus cannot be observed easily. Therefore, detection of morphological changes of the cells or nuclei induced by the virus before observing any later marked changes can be beneficial.
Methods: The cultured Vero cells were infected with rubella virus. The cells of the control and experimental groups were harvested at 2, 4, 8, 24, and 48 hours following the incubation period. The cells were processed and embedded in paraffin. Then, isotropic uniform random sections of the cells were obtained using ‘‘isector’’ method. Invariator, nucleator, and surfactor were applied to estimate the size of the Vero cells and their nuclei.
Results: The cell volume was decreased by 15-24% 48 hours after the infection in comparison to the non-infected cells. Besides, the cell surface area was decreased by 13% 48 hours after the infection. However, no changes were detected in the nuclei. The values of the standard deviation and coefficient of variation of the cells estimated by invariator were lower compared to those measured by the nucleator or surfactor.
Conclusion: Invariator is a more precise method compared to nucleator or surfactor. In this study, the volume and surface area of the Vero cells were reduced by rubella virus 48 hours after infection.
Keywords: Vero cells, Rubella virus, Histology
Estimation of the size of the cultured cells can be a valuable parameter in the in vitro culture studies. Up to now, a ...
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...th is the cell culture systems .15-16,20 In the present research, quantitative parameters were applied to detect rubella virus growth in Vero cell culture. This is a cheaper method for detecting rubella virus growth at the early stages of infection of the Vero cells as the cells which do not occasionally show gross morphological changes. It can also be useful for subsequent viral antigen preparation or serological testing of specific rubella antibodies followed by stereological confirmation of rubella virus growth in the Vero cell line.
The Vero cell volume and surface area can be decreased 48 hours after the infection determined using invariator, nucleator, and surfactor techniques. The values of the standard deviation and coefficient of variation of the cells estimated by Invariator were lower compared to those measured by the nucleator or surfactor