In Microbiology: An Introduction to Laboratory Procedures & Techniques (pp. 111-116). El Dorado, KS: McGraw-Hill Companies, Inc. Forrest, S., & Elliott, M. (2012). Lab 20 Identification of an Unknown Bacteria/ Published Results. In Microbiology: An Introduction to Laboratory Procedures & Techniques (pp.
So I started up with an alkaline solution that was not metabolically changed by the bacteria into an acid solution. Gas can also be produced as a positive result. Another test that was done was the triple sugar iron test. A loop full of the bacteria was transferred from the TSA plate to the broth; a spiral streak was carefully done to prevent poking the broth. A positive result changes to a dark color and a negative test did not.
It was determined using Thioglycollate agar deep the unknown culture was inoculated using a stabbing technique, and it was determined that the bacteria is facultative. The bacterium was also inoculated in glucose phenol red fermentation broth and lactose phenol red fermentation broth. Both tubes started red and the turned yellow if acid is present. So when the bacterium ferments it produces an acid and the phenol red turns yellow. There is also a small inverted tube that is placed inside of the tube which indicates whether gas was produced.
Microbiology is the study of microscopic organisms and has numerous applications in medicine, virulogy, immunology and more since the implementation of it in the lat 16th century. There are many microorganisms in the world habituating all kinds of conditions and locations, and the primary goal of microbiology to not only to identify but also characterize these populations. In the past this has been carried out by direct clonal culturing given the ease with which discoveries could be made about cultured organisms. This subsequently established a precedence for culture dependent isolations in the lab (1). However, as more evidence arose suggesting that this method only captures a small breadth of the microbial community, a new methodology has started to gain momentum.
The Catalase Test indicated a positive result. This showed that the bacterium contained the enzyme catalase which converts hydrogen peroxide into water and gaseous oxygen. The positive control used for this test was Micrococcus and the negative control for this test was Entercoccus. The Sulfur Reduction, Indole Production, and Motility (SIM) Test results indicated that the bacterium lacked the enzymes cysteine reductase or thiosulfate reductase and thus was unable to reduce sulfur. The positive control used for sulfur reduction was Proteus mirabilis and the negative control used was Staphylococcus epidermidis.
Based on the fermentation testes the microbe did ferment Glucose, Sucrose, lactose and Maltose. Si... ... middle of paper ... ...ll biochemical tests performed came out as expected except for the starch and urea digestion. On the first test starch shows a false + test and then on the second test it comes as a negative result. The same thing happened with the urea digestion it showed a false + test on the second test as a negative result, matching the result of a known bacterium, staphylococcus aureus.Therefore, it was concluded that unknown #79 was staphylococcus aureus. References Community college of Denver (2014).Introduction to microbiology laboratory manual.Boston,MA;person Learning solution Oxidative/fermentation glucose test.
During this test, the only solution that appeared to turn the flame any color was solution C, which turned the flame bright orange, indicating the sodium ion was present. This led us to the conclusion that solution C was sodium acetate. We next performed an ammonium test, which involved mixing our solutions with sodium hydroxide, and smelling the resulting solution in order to detect an ammonia smell. Solution B was identified as smelling like ammonia, indicating the presence of the ammonium cation. From this, we identified solution B as ammonium chloride.