Metabolization Of Methanotroph

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Methane is one of the major greenhouse gases responsible for global warming and scientists have been researching effective methods to metabolize methane and reduce its amount in atmosphere. Methanotrophs are bacteria that metabolize methane into mthanol with the help of an enzyme methane monooxygenase (MMO) and the genes encoding this enzyme have been identified. This gene can be cloned into some other non-methanotrophic bacteria which will metabolize methane through conditional protein splicing ie,only in presence of methane. This can be achieved by harnessing protein splicing; a posttranslational editing process that results in the precise removal of an internal domain (termed an intein) from two flanking sequences termed the N- and C-exteins. Here the protein splicing occurs only in presence of small molecule, methane. INTRODUCTION: One of the most dangerous greenhouse gases, methane is produced by all sorts of industrial and natural processes, including the decomposition of our own waste and that of livestock. Scientists fighting global warming are struggling to find ways to control the effects of methane, but one solution could come from a simple single-celled microorganism,methanotrophs. Methanotrophs are a group of gram-negative bacteria that utilize methane as their sole source of carbon and energy. The initial transformation involves the conversion of methane into methanol by methane monooxygenase (MMO). solubleMMO consists of three components, proteins A, B, and C. Protein A is made up of three subunits, a, b, and g, arranged in an a2 b2 g2 configuration, which are coded for by the mmoX, mmoY, and mmoZ genes, respectively, and is the hydroxylase component of the enzyme complex . Protein B acts as an effector of electro... ... middle of paper ... ...cientists to reduce the amount of atmospheric methane content. Mechanism by which methanotrophs convert methane to methanol can be very useful as it reduces atmospheric methane and the product methanol can be used as fuel source. The recombinant bacteria made with genes encoding the methane monooxygenase conditionally ie, in the presence of ligand can be useful in reducing pollution due to methane and the specificity of this enzyme producing bacteria can have various bioremediation application.Small-molecule-dependent inteins enable protein structure and function to be controlled posttranslationally in living cells. The development of small-molecule switches of protein function is a very active area of chemical biology, sometimes referred to as chemical genetics. The low efficiency of cloning techniques can be a difficulty to the achievement of recombinant bacteria.

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