In the light intensity experiment, tubes of buffer solution, chloroplast, water and DPIP were placed in different intensities of light to determine how light intensity affects the reaction rate of photosynthesis. The reaction rate was measured by absorbance values in five-minute increments for 30 minutes. The tubes were placed at 24 cm away, 30 cm away, 49 cm away, and completely dark. The tube that was closest to the light had the smallest absorbance value, while the dark tube had the highest value. As the light intensity increases, the absorbance value decreased. In the action spectrum experiment, 10 aspirated spinach leaves and sodium bicarbonate solution were mixed together in beakers then each beaker was placed in different light boxes.
The rate of photosynthesis can be determined indirectly by DPIP, which is a dye that essentially mimics coenzyme NADP, which becomes reduced when an electron is added to NADPH during light reactions (Lab manual, pg.114). When reduced the solution changes from blue to clear. When DPIP becomes clear, photosynthesis has occurred. Light Intensity can alter the photosynthetic rate of chloroplasts. At low light intensity, photosynthesis occurs at a slower rate due to the fact that only a small quantity of ATP is created(Slitcher). As light intensity increases, larger amounts of ATP and NADPH are created, increasing the rate of photosynthesis. Photosynthesis also requires a specific wavelength for the pigments in chloroplast to absorb it. Different pigments tend to absorb different wavelengths of visible light. Since pigments can only absorb certain wavelengths, the others are transmitted or reflected. The activity of photosynthetic pigments allows us to predict which pigments are responsible for photosynthesis at specific wavelengths. The purposes of our experiments were to observe whether or not light intensity and wavelength affect the rate of photosynthesis as well as find out what pigments were active at what point of
In our light intensity experiment our hypothesis was supported. The effect of light intensity is proportional to the photosynthetic rate. The absorbance values on the other hand had an inverse relationship to photosynthetic rate. Plants have pigments that pick up certain wavelengths of light. The pigment in the plant determines what wavelengths of light can be absorbed. Plants that are green normally contain chlorophyll a as well as b. These chlorophylls, absorb different types of wavelengths, but don 't absorb ones that are green. These plants are green because that wavelength of light is reflected instead of absorbed. Different colors of light can affect how much photosynthesis occurs, because only certain colors are absorbed. The more color that’s absorbed, the more light that’s absorbed, which leads to more photosynthesis. Our experiment demonstrated that the red wavelength was the most effective for photosynthesis behind white, which was our positive control due to the fact that it contains all the colors of the spectrum. In our final experiment for the absorption spectrum our results supported our hypothesis and the absorbance levels decreased as we increased the
This meant that this cuvette (tested under light) should display a higher decrease in DCIP due to the reduction in absorbance (dependent variable) opposed to the other cell fractions tested depending on a sixteen minute period (independent variable). The overall goal was to provide proof, through data, that the cell fractions put under the light during the sixteen minute period would indicate a higher set of chloroplast activity versus the ones put in the
As the light is increased so would the rate of photosynthesis. Apparatus: boiling tube, 250ml beaker, bench lamp, ruler, sodium
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
The Effect of Light Intensity on the Rate of Oxygen Production in a Plant While Photosynthesis is Taking Place
= > [CH2O} + O2 + H2O, This shows that when the light intensity is increased the rate of reaction will be more quicker he only anomalous result there was, is the one in the 100 watt result the reading after 5 minutes is anomalous because it does not follow the predicted pattern of increasing in the production of gas because it is lower I know from my own knowledge of photosynthesise that when the light intensity is increased the rate of reaction will be more quicker because many plants and trees photosynthesise quicker in stronger light and photosynthesise slower in dimly lit places. The chlorophyll absorbs light energy and enables it to be used by the plant for building up sugar. The overall effect is that energy is transferred from sunlight to sugar molecules.
In this laboratory experiment, the rate of photosynthesis was measured through the use of the “floating leaf disk technique.” The leaf disks were placed into a syringe and the O2 and CO2 in the mesophyll layers of the leaves were removed and then replaced with sodium bicarbonate or water, causing the leaves to sink to the bottom of the container. If one determines the number of leaf disks rising to the top as a result of an increase in oxygen gas in the mesophyll cells, then the rate of photosynthesis is able to be measured because O2 is a product of photosynthesis. The first step of this experiment was a feasibility study of the variance in the photosynthetic activity of the leaf disks in both water and bicarbonate solutions. After five minutes of light exposure, all of the leaf disks in the bicarbonate solution (10 disks) had ...
The Effect of Wavelength on Photosynthesis Rate Aim: To be able to To investigate how different wavelengths (colors) of light affect the photosynthetic rate of the synthetic. I will use a pant that is a pond weed called elodea. I will measure the rate of photosynthesis by measuring the amount of o2 given off in bubbles per minute from the elodea. I will do this by placing the Elodea in a test tube with sodium hydrogen. carbonate then I will vary the light wavelength (color) using colored.
The Effect of Light Intensity on Photosynthesis Of Elodea Canadensis Introduction I wanted to find out how much the light intensity affected the Photosynthesis in Elodea Camadensa. I decided to do this by measuring the amount of oxygen created during photosynthesis. Photosynthesis is the procedure all plants go through to make food. This process uses Carbon dioxide, water and light energy. It produces Oxygen and Glucose.
Increasing the light intensity will make photosynthesis faster. Variables: In this experiment there are a few things we have to keep the same.
Photosynthetic pigments are essential for life because they allow photosynthesis to occur by capturing sunlight which is then used alongside carbon dioxide and water to form organic compounds such as glucose and oxygen. The pigments allow the conversion of light energy to chemical energy which other organisms can benefit from. Oxygen is utilised by other organisms in aerobic respiration. The different pigments present in the chloroplasts allow a wide variety of wavelengths of light to be absorbed for efficient photosynthesis and provide colours to the plant to attract pollinators.
Light is a very important factor in the rate of photosynthesis, in my project I am going to test that plants do need light in order to photosynthesise. It will be very interesting to see how light will influence the rate of photosynthesis in plants and what will happen if they do not get the required light in order to produce starch .
This is the same if there is the light intensity is too high as this can damage the chloroplasts in plants and this will minimize the rate of photosynthesis. As shown in the graph as the light intensity increases, the photosynthesis rate increases until a point is reached where the rate begins to level off into a plateau. At a low light intensity, photosynthesis occurs slowly because only a small quantity of ATP and NADPH is produced. As the light intensity shown in the graph is gradually increasing, more ATP and NADPH (NADH is used in cellular respiration and NADPH is used in photosynthesis) are produced, which means more oxygen and sugar is produced, therefore increasing the rate of photosynthesis. But as the light intensity increases even more and past a certain light intensity on the graph, this is due to the other factors such as carbon dioxide limiting the rate of
The Effect of Light Intensity on the Rate of Photosynthesis in an Aquatic Plant Introduction The input variable I will be investigating is light, as light is just one of the 4 factors required in the green-plant process of photosynthesis. Photosynthesis is the process by which green-plants use sunlight, carbon dioxide, water & chlorophyll to produce their own food source. This process is also affected by the temperature surrounding the plant (the species of plant we experimented with, pond weed, photosynthesised best at around 20 degrees centigrade.) Light, temperature & CO2 are known as limiting factors, and each is as important as the next in photosynthesis. Light is the factor that is linked with chlorophyll, a green pigment stored in chloroplasts found in the palisade cells, in the upper layer of leaves.
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.
Increases in temperature can decrease the efficiency of photosynthesis; however the extent of this impact may be determine on the species' dependency on light.