Lab Practicum Report: Mucic Acid, Molisch
explanatory Essay
597 words
Vanessa Mendez Unknown# 26 Lab Practicum Report Results: The carbohydrate procedure was done due to the fact that my unknown represented a carbohydrate result by doing the Molisch Test. A TLC test was done to verify it was not an amino acid which proved to be negative. The Mucic Acid, Molisch, Bial's, Seliwanoff, Benedicts, and Barfoed's test was done to see the characteristics to help identify my unknown. Mucic acid test contained 100 mg of unknown, and 1 ml of HNO3 , and water which were heated for a hour. Bial's had 0.5 ml of unknown, and 1 ml of Bial's reagent heated to test for pentoses. Seliwanoff's tests for fructose or sucrose, by using 0.25 ml of unknown and the 1 ml reagent heating after. Benedicts test for monosaccharide's, …show more content…
In this essay, the author
- Explains that the carbohydrate procedure was done due to the fact that vanessa's unknown represented an amino acid. the mucic acid, molisch, bial, seliwanoff, benedicts, and barfoed'
- Describes the expected results of a carbohydrate-mucic acid mixture.
- Describes the results of the carbohydrate tests on the unknownmucic acid.
- Explains that the observed results matched the general expected results of galactose.
- Explains that the first test was the tlc test to verify that their unknown was not an amino acid, and then the molisch test, which was positive, was followed by the mucic test.
The observed results matched the general expected results of Galactose. Music acid formed a white precipitate. Bial's and Benedicts test had red precipitate. Seliwanoff test had no color. Bial's test had a dark brown color. Looking into the results obtained by doing the test Galactose was the adequate choice to what my unknown was. Discussion: Essentially my first test was the TLC test to verify that my unknown was not a amino acid which proved to be negative. After seeing this result I did the Molisch to test that the unknown was a carbohyrate. The Molisch test was positive which from this step the rest of the carbohydrate tests were done to find which carbohydrate it was. The Mucic test was the crucial one since it is only positive for Galactose, and lactose. When performing this test on my unknown it was positive. Seliwanoff had no color which served as a negative result for Fructose, and Sucrose. Bial's test was negative for a dark blue color which reflected that it wasn't Xylose. In Barfoed's test there was a positive red color which reflected that it was not lactose either. Looking at the data table with the observed results it made sense that the unknown was
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