Isolation of Nuclei and Mitochondria from Cauliflower Florets by Differential Centrifugation

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Isolation of Nuclei and Mitochondria from Cauliflower Florets by Differential Centrifugation

Nuclei and mitochondria are both organelles that are found within most

eukaryotic cells. The nucleus contains most of the genes needed for

classification. It is "one of the most prominent structures to be

encountered in the eukaryotic cell" (Schwarz 24). Nuclei were first

observed by a Scottish plant taxonomist name Robert Brown in 1831. He

studying Orchidaceae and Asclepiadaceae at the time when he noticed a

structure in the cell that was consistent with much of the cells he

was viewing. He termed this "the nucleus" (Enersen 1). Later in the 19th

century scientists started using dyes to stain the nucleus. It was

found to be consistent in plant cells as well as cellular division

(Robert 1). The nucleus is enclosed by a double membrane nuclear

envelope. Inside the nucleus DNA is organized into material called

chromatin (Biology 117). Nuclei are the central part of the cell, the

densest organelle, and considered to be the brain of the cell.

Mitochondria are believed by many to be the result of a symbiotic

relationship between a bacteria and a primitive cell. This theory is

called "the endosymbiont hypothesis" (Schwarz 24). Some believe that

early bacteria invaded or were engulfed by a cell and instead of being

digested the bacteria formed a symbiotic relationship. Mitochondria

are involved in the catabolic process that generated ATP from sugars,

fats, and other forms of fuel. ATP, adenosine triphosphate, is an

organism's energy source. Living things convert fuels into ATP which

is handled easier by the creature. Mitochondria are enclosed in a

...

... middle of paper ...

...s feasible. The process of

differential centrifugation describe above is a worthy procedure to

use if small particles are needed to be isolated and observed. This

experiment illustrates how this process can be carried out as well as

provides data found on completion.

Works Cited

Biology (6th ed.). ed. Neil A. Campbell and Jane B. Reece. San

Francisco: Benjamin Cummings, 2002.

Enerson, Daniel Ole. "Robert Brown." Who Named IT?

. 1994-2001.

"Robert Hooke Describes Cells."

.

Schwarz, Otto J. "Introduction to Basic Laboratory Skills for the

Biological Sciences- An Experimental Approach." Oak Ridge, TN:

Performance Press, 2000.

Figure 1 Nuclear Diameters

µm

Text Box: Nuclei observed[IMAGE]

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