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Effect of osmosis on plant tissue variables
Effect of osmosis on plant tissue variables
Effect of osmosis on plant tissue variables
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Investigation of Osmosis in Plant Tissue
Aim
To investigate factors which affect the rate of osmosis.
The main factors which affect osmosis are:
Pressure
Temperature
Surface area
Solute Concentration
Plant Tissue
We will be investigating solute concentration as this is the easiest
factor to measure and will give us the most accurate results. We will
mainly investigate the different rate of osmosis when potato cells are
placed in solution with different solute concentrations. We are going
to use potatoes as they have homologues cells (all their cells are the
same).
Preliminary work
We investigated the rate of osmosis of potato cylinders left in
different solute concentrations.
Our hypothesis was that Rate of osmosis is proportional to the
difference in water potential inside and outside the cell.
Water potential(y) indicates which way water will move in a system.
Water will always move from a high y to a low y. y can be measured in
Kilopascals (kPa). Pure water has a y of 0 kPa. As solute is added, y
is reduced (it becomes a negative number.)
e.g.
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This is useful because you can add pressure to the equation.
If a pressure of 400 kPa is added to B (due to squeezing), the
equation is
-1000 + 400 = -600
-600 = -600
Now that the forces are equal, there will be no water movement.
We will measure y using Molar Concentration which has a concentration
of 0 - 1. Pure water has a y of 0M.
Using ’Biology, a functional approach’ by MBV ROBERTS, we deduced that
the y of a potato cell º 0.27M.
The water potential inside the cell is 0.27 M. I predict that there
will be the lowest rate of osmosis at this point and it will get
higher as the solute concentration levels move further away from
0.27M. The rate of osmosis will be higher when there is a greater
difference in water potential.
* pH of Solution: Water has pH of 7 at 25°C. As the pH decreases,
Theory of Water of Displacement: A volume of water was measured. A second volume of water was measured when the metal cylinder was added. The initial volume was subtracted from the second (total) volume to get the volume of the metal cylinder.
I also predict that solution concentration 0.3 will be isotonic because of the pressure potential. As the water moves into the cell it pushes up against the cell wall this is called the pressure potential and it increases the water potential until an equilibrium is reached, and because the cell is so inelastic it takes very little water to achieve this. That's why I believe in-between 0.3 and 0.4 will be isotonic as it is very close to pure water. Background Information Osmosis - The net movement of water molecules from a region of high water potential to a low water potential, through a selectively
Solutions have three different stages that the solutes can be classified in: isotonic, hypertonic, and hypotonic. Isotonic is when the solutions have equal amounts of solutes. Like equilibrium, there is no net change in the amount of water in either solution. When the solutions have different concentration of solutes then the one with less solute is hypotonic and the one with more solute is hypertonic. Hypotonic takes in the solute from the hypertonic side that gives away the solute.
* Note the mass down in the table at the end of the first page.
Conclusion: Finally, water molecules are moved from high concentration to low concentration Based on the data collected and the results of the experiment, the hypothesis was correct.
The 100% solution is sodium chloride dissolved in water (salt water). This will be referred to as the 100% solution from now on. But the type of solution is not important as osmosis is all about differences in concentrations. The output variable will be the weight of the potato chips, the movement of the water into and out of the potato. Osmosis is the diffusion of water through a semi-permeable membrane from high concentration of water to low concentration of water.
the same way as it does potato. I would also widen the range of sugar
Osmosis in Carrots Background Osmosis is the diffusion of water from a dilute solution to a more concentrated solution through a partially permeable membrane, which allows the pass of water molecules but not solute molecules. [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE]If a cell is placed in a less concentrated solution water enters because the less concentrated solution will have a high concentration of water than the inside of the cell. Once the cell takes in maximum water the cell becomes turgid. If the cell was to be placed in a high concentrated solution, water would leave the cell because the cell would contain a low concentrated solution. So in the low concentrated solution there will be a high concentration of water and in the high concentrated solution there will be a low concentration of water.
Water that has changed throughout the hydrologic process travels from the irrigation treatment and enters into the aquifers. First...
When I am not using them I will place them away from my experiment and
Temp: Mass of evap. dish: Mass of evap dish+contents: Mass of contents: Solubility g/100cm3 water
water in the potato, then the water will go out of the potato and into
Part A of the experiment, we were measuring the density of water. In this part, we measured by difference by measuring the mass of the empty graduated cylinder which was 46.35 grams and then added 25.0 milliliters of water to it. When subtracting by difference, our mass of the water was 25.85 grams. This was close to the measurements of the water added to the graduated cylinder. The density of the water was 1.0 grams/milliliters.
g. of KI in 10 mL of water. Add the KI solution dropwise to the test