Investigation of Factors Affecting the Rate of Breakdown of Hydrogen Peroxide by Catalase
Certain reactions in the cell produce hydrogen peroxide, which is
poisonous. The enzyme catalase is able to render it harmless producing
water and oxygen.
Prediction
I predict that as the concentration of hydrogen peroxide increases, so
will the amount of oxygen released in a given time. This is because in
an increased concentration of hydrogen peroxide there are molecules to
come into contact with the catalase and so the reaction happens
faster.
Input Variables
· Temperature
· Concentration of hydrogen peroxide
· Volume of hydrogen peroxide
· Amount of catalase
· Surface area of enzyme
Output Variables
· Amount of oxygen
· Amount of water
Plan
The equipment will be set up as above in order to find the results.
The measurements to be taken are: 1.25M; 1.00M; 0.75M; 0.5M; 0.25M,
which will each be collected three times and then averaged. This is so
that I will have a good set of results to come to a conclusion with.
From preliminary work that I had previously carried out I have found
some useful things that will help in this experiment. These are that
the size of the catalase has to remain constant in all of the
experiments. This is so that it is a fair test, as well as not making
the catalase to big or to small because the reaction may occur to
slowly or to quick.
Diagram
Risk Assessment
Whilst doing the experiment there are various risks that have to be
taken into consideration. The biggest one of these is that hydrogen
peroxide is dangerous because it is poisonous. This will be dealt with
by wearing safety goggles and keeping the room well ventilated.
Scientific Theory
The reaction being carried out can be explained by using collision
theory and enzyme theory. For a reaction to occur there must be
collisions between the molecules and activation energy is produced
to get an idea of how I would do my real experiment and what apparatus
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Investigating Factors that Affect the Rate of Catalase Action Investigation into the factors which affect the rate of catalase action. Planning Aim: To investigate the affect of concentration of the enzyme catalase on the decomposition reaction of hydrogen peroxide. The enzyme: Catalase is an enzyme found within the cells of many different plants and animals. In this case, it is found in celery.
What Affects the Rate of Breakdown of Hydrogen Peroxide by Enzymes Aim = == The aim of this experiment is to find out how temperature and concentration affect the breakdown of hydrogen peroxide by an enzyme (yeast). I hope to achieve reliable results that will confirm my predictions.
The Effect of a Catalase on the Breakdown of Hydrogen Peroxide Aim To follow the progress of a catalysed reaction by measuring the volume of gas produced as the reaction proceeds. Using the initial rates of a series of experiments I will be able to find the orders of the reaction with respect to enzyme and substrate. Also to find out if concentration has an effect on the reaction when an enzyme is used to accelerate the breakdown of hydrogen peroxide.
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
The Effect of Surface Area on the Rate of Reaction Between Catalase from a Potato and Hydrogen Peroxide
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
== = == Hypothesis for the experiment: After I conduct this experiment, I expect and suppose I can recognize and physical changes, identifying the difference these two kinds of changes. Also, I will be able to know some physical and chemical properties of copper (II) sulfate, water, iron, sodium carbonate, hydrochloric acid and magnesium and identify if it is a chemical change or physical change in each part of the experiment.
List of the tests to be conducted, material to be tested, the location of sampling, the organization’s name that will perform the test, and the frequency of testing.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
Planning and Method. Any experiment needs variations as well as fairness to be a true success. The two variations I will include in this experiment shall be: 1) The number of paperclips on the bottom of the helicopter. 2)