Investigation Into The Effect Of Changing The Substrate Concentration On The Enzyme Catalyse

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Investigation Into The Effect Of Changing The Substrate Concentration On The Enzyme Catalyse Introduction ============ I am experimenting the effect of substrate concentration on the action of the enzyme catalyse. I will investigate this by experimenting to see how long it takes to fill a test tube full of the gas from the reaction, which is oxygen. I will use different amounts of catalyse in the form of yeast to test how long it takes to fill the test tube with oxygen, which is one of the products from the reaction. Background Info Enzymes are biological catalysts. A catalyst will lower the Activation energy of a reaction therefore allowing the reaction to work at lower temperatures and/or pressures then the reaction without the catalyst. An enzyme can take two substances which we call substrates and either bond them into one or, break them down into two. This can be called the 'lock and key.' This is shown in the diagram below: Enzyme action The catalyse that we will be using is yeast. This will react and break down the hydrogen peroxide (H2O2) to give us Oxygen and water (H2O and 02.) The hydrogen peroxide and yeast should react quicker if the hydrogen is at a higher concentration. This is because when the H2O2 is at a higher concentration, there is more particles. This means that the yeast and H2O2 particles collide more frequently, forming more enzyme complexes. Therefore the reaction will take place faster. Method · Take a test tube of water and place it, upside down into a basin of water, of a suitable size. · Put 20 cm3 of diluted hydrogen peroxide in a conical flask. · Put a rubber bung into the top of the conical flask, with a delivery tube perturding out of the top, and into the basin of water, making sure that the other end of the delivery tube is just below the water surface. · Place the upside down test tube full of water over the delivery

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