Investigation Into The Effect Of Changing The Substrate Concentration On The Enzyme Catalyse
Introduction
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I am experimenting the effect of substrate concentration on the action
of the enzyme catalyse. I will investigate this by experimenting to
see how long it takes to fill a test tube full of the gas from the
reaction, which is oxygen. I will use different amounts of catalyse in
the form of yeast to test how long it takes to fill the test tube with
oxygen, which is one of the products from the reaction.
Background Info
Enzymes are biological catalysts. A catalyst will lower the Activation
energy of a reaction therefore allowing the reaction to work at lower
temperatures and/or pressures then the reaction without the catalyst.
An enzyme can take two substances which we call substrates and either
bond them into one or, break them down into two. This can be called
the 'lock and key.' This is shown in the diagram below:
Enzyme action
The catalyse that we will be using is yeast. This will react and break
down the hydrogen peroxide (H2O2) to give us Oxygen and water (H2O and
02.) The hydrogen peroxide and yeast should react quicker if the
hydrogen is at a higher concentration. This is because when the H2O2
is at a higher concentration, there is more particles. This means that
the yeast and H2O2 particles collide more frequently, forming more
enzyme complexes. Therefore the reaction will take place faster.
Method
· Take a test tube of water and place it, upside down into a basin of
water, of a suitable size.
· Put 20 cm3 of diluted hydrogen peroxide in a conical flask.
· Put a rubber bung into the top of the conical flask, with a delivery
tube perturding out of the top, and into the basin of water, making
sure that the other end of the delivery tube is just below the water
surface.
· Place the upside down test tube full of water over the delivery
We used the pipette filler and filled the glucose rinsed pipette to add 10ml of 10% of glucose in test tube 0.
3. The beaker was filled with water and the metal was placed in the water.
For this experiment, you will add the measured amount of the first sample to the measured amount of the second sample into its respectively labeled test tube then observe if a reaction occurs. In your Data Table, record the samples added to each test tube, describe the reaction observed, if any, and whether or not a chemical reaction took place.
the food sample on fire, once it catches, immediately place the small chamber on top with the rod,
Investigation of How the Concentration of Catalase Enzyme Affects the Rate of Reaction Aim: To find out how the concentration of Catalase Enzyme will affect the enzyme activity and the rate of reaction towards Hydrogen Peroxide. (H O ) Prediction: I predict that with the higher concentration of enzyme, the likelihood of it breaking down molecules will be greater because there will be more enzymes to work at the substrate and the chances of it colliding will be higher making the activity time quicker. Equipment: · Syringe · Measuring Cylinder (×2) · Knife · Blender · Beakers (×2) · Balance · Hydrochloric Acid in a beaker · Stop clock · Potato · Water in a beaker Preliminary Experiment: In this experiment we will be using an enzyme called Catalase. By using different amounts of this enzyme we will be diluting it with water to test how the concentration of Catalase affects the rate of reaction with Hydrogen Peroxide.
The Effect of Changing the Concentration of the Enzyme Catalyst on the Rate of Reaction on Hydrogen Peroxide
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
2. Lift the Ball Bearing to a height of 10cm and drop into the tub of
3. Add on of the following volumes of distilled water to the test tube, as assigned by your teacher: 10.0mL, 15.0mL, 20.0mL, 25.0mL, 30.0mL. (If you use a graduated cylinder, remember to read the volume from the bottom of the water meniscus. You can make more a more accurate volume measurement using either a pipette or a burette.)
I am measuring how long it takes to collect 10 cubic cm of gas in a
Investigating a Factor that Affects Enzyme Activity Planning -------- Aim --- To investigate a factor which will affect the activity of catalase, whilst keeping all variables constant. Possible Independent Variables ------------------------------ Here are a number of possible independent variables that could be changed in the experiment: Independent variable Continuous/Discontinuous Easy to measure?
2. A test tube was then filled with 35ml of yeast and placed in the
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
We attached a gas syringe via a plastic tube to a test tube and used a
Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide Introduction The aim of this experiment is to determine the effects of varying enzyme (catalyse) on Hydrogen Peroxide. Hydrogen Peroxide + Catalyse à Water + Oxygen 2H2O2 à H2O + O2 + Heat Apparatus & Diagram [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE] Bung Potato Hydrogen Peroxide Water Collected Oxygen Delivery Tube Measuring Cylinder [IMAGE] Using the Equipment Safely It is important that we use the apparatus carefully, as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes. Catalyse is an enzyme found in all living cells.