Investigating if the Concentration of CuSO4 Affect the Germination of Barley Seeds

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Investigating if the Concentration of CuSO4 Affect the Germination of Barley Seeds Investigation ============= Analysis -------- When looking at the data collection sheet it can be seen that as the concentration of CuSO4 increases, the percentage of seed germination decreases. This already shows a clear sign that enzyme inhibition has occurred. Looking at the “Average Length of Roots” graph, it can be seen that the concentration of CuSO4 does inhibit the growth of roots significantly because the average root length drops sharply after the 0% solution. However some of my results nay have been anomalous because the decline is far too steep to draw any reliable conclusions. When comparing this graph to the “Average Length of Shoot” graph, it can be seen that the average shoot length for each concentration depreciates at a steadier rate; perhaps then, heavy metal ions affect enzymes that are responsible for root growth more than they affect those that are responsible for shoot growth. This is could be a topic for further investigation. For both graphs however, its shows that the depreciation rate between each concentration decreases. For example, using the “Average Length of Roots” graph it can be seen that the gradient between each concentration becomes steadier and steadier as the concentration increases. This shows that inhibition of growth is affected most directly after the 0% concentration. When looking at the average mass of roots, we can see that it falls from 0.98g to 0g from 10% copper sulphate solution to 0% concentration. There is a substantial drop between 0% Copper Sulphate and 10% showing that on average the best rate of growth for a seed is without the presence of Copper Sulphate. Conclusion In my hypothesis, I stated that as the concentration of copper sulphate solution increased, the level of seed germination would decrease. I feel that my results clearly show this and prove my hypothesis correct. The reason for this is because the enzymes used for germination (amylase, maltase, lipases, proteases etc) are

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