Investigate the effect of temperature change on the rate of hydrolysis

Investigate the effect of temperature change on the rate of hydrolysis

of starch using the enzyme amylase.

Amylase investigation

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AIM: To investigate the effect of temperature change on the rate of

hydrolysis of starch using the enzyme amylase.

These are variables that may affect the rate of the reaction:

* concentration of amylase

* concentration of iodine

* temperature conditions of reaction

* pH conditions of reaction

I intend to investigate the effect of temperature change on the rate

of the hydrolysis of starch, using the enzyme amylase.

In order to do this while also ensuring a fair test, I shall maintain

all other latent variables constant.

PREDICTIONS: I expect that the time taken to evolve a set volume of

gas will decrease as the concentration increases. This, in other

words, means that to increase the concentration, will increase the

rate of the reaction.

I would expect that if the concentration is doubled, so too is the

rate of the reaction:

Rate of reaction µ concentration

JUSTIFICATION: Enzyme molecules have a very specific method of

converting those molecules upon which they work (the substrate

molecules).

Enzyme molecules are proteins that act as biological catalysts, so

they are not themselves used up when converting substrate molecules.

On every enzyme molecule is an active site where the substrate

molecules are joined to the enzyme molecule, and converted to produce

molecules.

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