In Vitro Propagation and Assessment of Clonal Fidelity of Dendrobium Aphyllum

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Orchids belong to the family Orchidaceae one of the largest and most diverse groups among angiosperms. According to one estimate, the family Orchidaceae includes 800 genera and 25,000 species (Stewart and Griffiths 1995). These plants are outstanding in many ways as they have diverse shapes, forms and colors. However, due to heavy rate of deforestation and other anthropogenic pressures the natural populations of many splendid orchids are depleting fast from their natural habitats at an alarming rate and their conservation is becoming a matter of global concern. Presently, all the orchid species are indexed in the Red Data Book of International Union of Conservation of Nature and Natural Resouces (IUCN). Also, the family Orchidaceae is now included in Appendix-II of Convention of International Trade in Endangered Species of Wild Fauna and Flora (CITES) (Senthilkumar 2001).
The use medicinal use of orchids in treatment of disorders and ailments dates back many centuries and was reported by the Chinese (Bulpitt 2005). The Chinese pharmacopoeia, “The Sang Nung Pen Tsao Ching’’, illustrated Dendrobium as a source of tonic, astringent, analgesic, and anti-inflammatory compounds as far back as 200 BC and since vedic times (Singh and Tiwari 2007). Orchids are used in traditional medicine as they are rich in active compounds including several alkaloids (Okamoto et al. 1966; Lawler and Slaytor 1969; Elander et al. 1973; Nurhayati et al. 2009). Being one of the main constituents of “Herba Dendrobii”, Dendrobium aphyllum deserves special mention. The phenolic constituents of D. aphyllum comprised nine bibenzyls and two benzylethanyl compounds of which moscatilin and moscatin are also present (Ng et al. 2012). The paste of D. aphyllum is used...

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