Growth modulatory effect of Costus speciosus rhizome extracts on MCF-7 human breast cancer cells
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Naturally derived products, such as the plant extracts or their pure compounds are the major possessions that stand ahead in drug discovery, with their property of novel identity with that of the available chemical diversity (Cosa et al., 2006). Form the various cancer chemotherapy progresses of the past decades, the plant natural products had been found to be a vital source in contributing to the launch of cancer drugs in the market (Cragg et al., 1997). The importance of plant derived drugs and its implication in curing disease has made the interest in the identification of new plant sources for the management of cancer.
The in vitro viability assay of rhizome extracts of C. speciosus on MCF-7 cells proved a dose-dependent decrease in the percentage of cell’s viability in all the extracts treated cells when compared to the control [Fig 1]. The hexane extract showed a significant decrease at a very low concentration, with 50% inhibition in the cell viability at 10 µg/mL, whereas the ethyl acetate and methanol extracts had 50% inhibition of viability at 25 µg/mL and 15 µg/mL concentrations respectively. These concentrations are the IC50 values of the particular extracts and can be used for the further studies on the extract. The maximum inhibition at a minimum concentration made the choice of hexane extract (HE) for the further studies. Plant molecules are pharmacological agents that enhance the ability to induce programmed cell death. Several herbal alkaloids act as antiproliferation and anti-metastasis stimulators (Jin et al., 2012). Plant flavanoids and terpenoids inhibit cancer cell proliferation, cell cycle arrest and apoptosis via various mechanisms (Maheep et al., 2011; Huanjie et al., 2010). Thus the prese...
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...interaction of costunolide, eremanthin and 5-HMF present in the C. speciosus hexane extract with the cancer target Bcl-2 [Fig 4a, 4b, 4c]. The best receptor-ligand complex was chosen based on the criterion of its energy and score values that ranked first of all the poses obtained. A stable receptor-ligand interaction is sustained with the presence of hydrogen bond interaction with a bond distance less than three (Daisy et al., 2012). Similarly all the interactions of the study had hydrogen bonds with a bond length less than three, confirming the stability of the interaction [Table 2]. This study predicts the target action of costunolide and 5-HMF in fit with Bcl-2 and a similar action of eremanthin predicts its role too as an apoptosis inducer. This prediction of the action of the phytocompounds on Bcl-2 towards cell apoptosis helps in the further molecular studies.