Glycolysis Process Of The Krebs Cycle Experiment

1166 Words5 Pages
Abstract In this experiment, a liver extract solution was obtained from a freshly killed female white mouse by placing in a 100 ml sucrose-Tris phosphate buffer, homogenized, and separated by centrifuged. The extract was mixed in two tubes with different components, one with buffer, succinate, liver extract, and the dye 2,6-dichlorophenolindophenol (DCIP) - the control tube. The second tube with these solutions in addition to cyanide –experimental tube. The rate of electron transport and the effect of cyanide on the rate of reduction 2, 6-dichlorophenolindophenol, was measured and tested. It was concluded that the rate of reduction of oxidized DCIP increased with the addition of cyanide because cyanide disrupts or poisons the electron transport…show more content…
Pyruvate, the three carbons molecule that formed from the glycolysis process initiates the start of the Krebs cycle. In this cycle, NADH is produced in to provide the electrons needed for the mitochondrial electron transport. NADH start the cycle and the final acceptor is oxygen. No final electron accepter (O2), leads to reduction and the production of ATP stops (Dr. Laurates, Bio213, LecWeek2Notes.pdf). The conversion of Succinic acid to Fumaric acid occurred in the mitochondria matrix so that succinate is available to produce…show more content…
The dye involves with the electron transport system and competes for electrons therefore the dye get reduced. (Dr. Bisson, Bio213 RecWeek1Notes.pdf). When DCIP is reduced, the color will change from blue to colorless. Reduction and oxidation depends on the redox potential for each molecule. NADH is a better electron donor because it has a lower reduction potential than FADH2.Two test tubes will have the solution and dye without cyanide but one will have cyanide added to it. Electron carrier, cytochrome c, transports electrons from the second hydrogen ion pump to the third. However, cyanide combines with iron and inhibits cytochrome c from transporting electrons to oxygen in the fourth complex of the electron transport chain (Dr. Laurates, Bio213, LecWeek2Notes.pdf). Therefore the electron transport chain is disturbed causing the aerobic production of ATP to stop and the DCIP takes up all the electrons because the electron chain is backup with all these electrons. As a result, one can concluded that the addition of cyanide will increase the rate of reduction of oxidized
Open Document