Genetic Dna And Its Effects On A Patient 's Body Through A Variation Of Technique Under Gene Therapy

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Largely, the mammalian heart possesses a capacity for renewal that is insufficient for regeneration in response to injury but that might be enhanced by therapy. Moreover, it has been proved (through recombinant DNA techniques) that paracrine signaling between epicardium and myocardium regulates vascular endothelial dysfunction as well as cardiomyocyte proliferation and differentiation for cardiomyopathy. Methods: There are several molecular techniques that are effective in obtaining regenerative heart tissues. By manipulation of genes that encode for specific proteins and/or transcription factors, a change in phenotype can be produced. Recombinant DNA is used for cloning (i.e. making copies of genes). An example that demonstrates this is, Eli lily and insulin. Lily the first to market insulin which was made by recombinant DNA technologies and is trademarked under the name humlin today. All of the discussed techniques below can potentially reduce the effects of heart disease on a patient’s body through a variation of technique under gene therapy. Recombination and Cardiac arrhythmias: For cardiac arrhythmias physiological abnormalities are credited for increasing the risk of life-threatening arrhythmias. Stated previously these diseases evolve through a defect in the potassium ion channels. This could be fixed with recombinant DNA methods, or the insertion of a mutated gene through site-specific recombination creates a transgenic mouse. Transgenic animals usually provide scientists a way to replace a perfectly good gene with a mutated one. In this case, the opposite is perform where replacing the problem gene will pave the way for future advances for curing cardiac arrhythmias. Cardiac arrhythmias requires a defective gene r... ... middle of paper ... ...e knockout DNA to transfect the donor cells. Inject the ES inside a normal blastocyst cell from the normal mouse. Implant the blastocyst into a foster mother (usually the donor of the embryonic blastocysts) to generate a chimeric mouse. Take the chimeric mouse and cross it with a normal unaffected mouse to generate a successful mouse with that is symptomatic. A symptomatic mouse should help aid the identification of the source within the defective gene. Overall, the suggested techniques above have the potential to pave the way for advances of regenerative medicine for heart related diseases. Finding the molecular source of these diseases can ultimately find the cure instead of harming the patient’s body with corrective surgeries that have a high rate of restenosis occurring. Heart disease is a huge killer worldwide and finding the root of the problem is crucial.

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