Cloning
Cloning is a process that creates exact genetic copies of an existing cell.Cloning is a more general term that describes a number of different processes that can be used to produce genetically identical copies. The process of cloning can happen either naturally, for instance, when identical twins develop or it can be induced through synthetic conditions in a laboratory. There are three different types of artificial cloning: gene cloning, reproductive cloning and therapeutic cloning.
Gene cloning works by first isolating the desired gene and ‘cutting’ it from the original chromosome using restriction enzymes. The piece of DNA is ‘pasted’ into a vector and the ends of the DNA are joined with the vector DNA by ligation. The vector is introduced into a host cell, often a bactera or yeast, by a process called transformation. The host cells copy the vector DNA along with their own DNA, creating multiple copies of the inserted DNA. The vector DNA is separated from the host cells’ DNA and purified. Gene cloning is used to create a large number of copies of a gene. The cloned DNA can be used to decipher the function of the gene, Investigate a gene’s characteristics like size, or expression, look at how mutations may affect a gene’s function or make large concentrations of the protein coded for by the gene.
Reproductive cloning is a type of cloning which is performed for the purpose of creating a duplicate copy of another organism. It creates exact genetic copy, or clone, of an individual. It is accomplished using a process called somatic cell nuclear transfer(SCNT). SCNT when done on a mouse for instance, functions by taking the nucleus out of a somatic cell(any non gamete cell) of one mouse and then that nucleus is put into ...
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...y into a cell it usually will not function the desired way. Instead, a carrier called a vector is genetically engineered to deliver the gene. Certain viruses are often used as vectors because they can deliver the new gene by infecting the cell. The viruses are modified so they can’t cause disease and will not be fought off by the patient’s immune system. The vector can be injected or given by IV directly into a specific place in the patient’s body. The vector can also be introduced after , a sample of the patient’s cells are removed and exposed to the vector in a lab. The cells with the vector are then returned to the patient.
In conclusion, all three of these practices offer the opportunity for great medical advancement but struggle with ethical issues and possible risks as well as questions of their viablity as legitimate treatments to benefit medical problems.
However, I believe the choice is up to the individual receiving treatment to decide the method of their treatment in accordance with their beliefs. I suppose my stance with this is strongly rooted in my acceptance of stem cell research, despite which method is used. While the use of adult cells is less controversial, I do not think they have the capacity to completely replace the use of embryonic stem cells without more testing. Although, I personally am indifferent to where the cells come from, I do not condone effecting an abortion for the sole purpose of stem cell therapy or cloning one child to have the clone just as a lifetime organ donor. I would prefer that the stem cells used for testing be taken from medical waste or otherwise discarded materials. Perhaps one day the stem cells received from adults or the umbilical cord will satisfy all needs and possibly put an end to controversy (NIH,
Either transduction or transfection can be used to get the therapeutic genes into the patients system. Transfection is when the genes are introduced physically or chemically in a way that allows the cell membrane to be temporarily permeable to a foreign DNA. In the second method used for gene therapy, transduction, there is a beneficial gene added into the genetic material of the virus, which then is allowed to infect the target cell which is the indirect transfer method for gene therapy.
Cloning is a real process that scientists use today to reproduce an exact living copy of DNA from the DNA of another living organism. When the idea of cloning first came about in the early 1800’s people believed it to be more science fiction than actual science. People didn’t understand the concept of cloning and therefore was naturally scared of the subject. It is best understood by how the Department of Animal Sciences at Cornell University explains it, “Cloning is a method of producing two or more genetically identical organisms by asexual reproduction. This means that there is only one parent cell, from which all the genetic information will come. Thus, the DNA sequence of cloned organisms is exactly the same as that of the parent cell.” Despite the general population’s disbelief there have been major scientific advances in the cloning process in the last fifty years. After many years of trial and error the first successful clone was created.
Gene therapy is an experimental technique that allows doctors to insert a gene into a patient’s cell rather than using drugs or surgery. Gene therapy is a process of which defective or undesired genes in the body with “normal” genes. A vector is re-engineered to deliver the gene to a target cell. Then the gene is transferred to the cell’s nucleus and must be activated in order to function. The main focus of gene therapy is to replace a lost or improper gene with a new functional copy into a vector that is inserted into the subject’s genome by way of penetrating its DNA. Gene therapy can be done outside of the body known as ex vivo by way of taking cells form patients bone marrow or blood and then growing them in a laboratory. Thus the corrected copy of the gene is inserted into the cells before being put back into the body. Gene therapy can also be done in vivo which can be done directly to the patient’s body. The word gene therapy really defines the management of genetic information that is encased in the cells, however, in most recent procedures the available technology is closely related in adding new genetic information, and many researchers favor the term gene transfer rather than gene therapy to mirror the reason that the purpose of gene work cannot always be therapeutic.
Although the highly technical aspects of human gene therapy are somewhat complex, the basic concept is very straight forward. The goal of gene therapy is to correct mistakes that have occurred within the genetic material, or DNA, of the living cell. In very simple terms, DNA is often thought of as the "language" of the biological functioning of organisms. This language is organized by letters (nucleotide pairs), words (codons), sentences (genes), and books (genomes). Before being able to repair the damaged or defective genetic material, the location of the gene or genes causing the dysfunction in the individual must be determined.
Gene therapy works in three ways; it works to replace a missing or defective gene with a normal one, replace a faulty gene so that it will function properly and it works to activate and deactivate a gene, allowing it to “switch” on and off. Gene therapy is done by the deliverance of a gene to a cell via a carrier, or vector, such as a virus. Scientists lean more towards using a virus because they can seek out particular cells and transfer pieces of deoxyribonucleic acid into them. Scientists also take advantage by deactivating their harmful characterizes and modifying them to carry particular gene into designated cells. After gene therapy is done, the genes can then stimulate the production needed for standard functioning, allowing that gene to return to its previous normal state. Therefore, if a patient were to be in the beginning stages of cancer, gene therapy would seek out the cancerous gene and replace it with a healthy one and minimizes the disease from
Gene therapy gives people who suffer from genetic diseases a chance to lead a normal life. Dangerous diseases, such as AIDS, SCID, Thalassemia and ADA can be cured successfully. In September 5, 2006, two people with advanced melanoma received Gene therapy and they got recovery soon. This is a breakthrough in cancer gene therapy. Gene therapy uses patients own cells to cure diseases, and, therefore, no rejection to their bodies. Furthermore, patients could get permanent cure from gene therapy without recurrence.
First and foremost, it is important to discuss what human cloning is. It is the conception of in vitro embryos that produces “individuals that are exact genetic copies of the donor from whom the DNA was obtained” (Munson 366). In Laymen’s terms, cells are inserted from the donor host into an unfertilized egg from another host (meaning it is asexual) and the new egg is transferred into the surrogate mother where it will foster into an embryo, if effective.
...us or bacterium used as the carrier. Recombinant vector vaccines are still being experimented on today, just like the DNA vaccinations. The only difference between recombinant vector vaccines and DNA vaccines is the fact recombinant vector vaccines use an attenuated virus or bacterium to introduce microbial DNA to the body’s cells. Scientists created this vaccine by observing nature, and how nature passes viruses along. Scientists noticed that viruses in nature latch on to the cells they want to inject, this caused scientists to figure out how to take parts of an attenuated virus and add genetic matter from other microbes into them. I know this sounds confusing, but it’s quite simple. Just think of it as poisoning the virus. Recombinant vector vaccines are very close to mimicking a natural infection, which causes the immune system to energize and start up sooner.
First, what exactly is cloning? In biology, cloning is used in two contexts: cloning a gene, or cloning an organism. Cloning is the reproduction of a human or animal whose genetic substance is identical to an existing being, such as an embryo or fetus. This is reproductive. Cloning a gene means to extract a gene from one organism and insert it into a second organism. Cloning an organism means to create a new organism with the same genetic information as an existing one. This is therapeutic.
Stem cells offer exciting promise for future therapies, but significant technical hurdles remain that will only be overcome through years of intensive research. Stem Cells have the incredible potential to develop into many different cell types in the body during early life and growth. Scientists primarily work with two kinds of stem cells from animals and humans. The embryonic stem cells and the non-embryonic stem cells. Stem cells are the cells from which all other cells originate. In a human embryo, a large portion of the embryo’s cells are stem cells. These stem cells can be used for cell-based therapies. Cell-Based therapies are treatments in which stem cells are induced to differentiate into the specific cell type required to repair damaged or destroyed cells or tissues. Stem cells are versatile and offer the possibility to treat a number of diseases including Alzheimer’s, stroke, cardiovascular disease, diabetes, etc. The problem is that for the process of embryonic stem cell research and embryo will be destroyed if used. This raises a moral issue and questions of whether stem cell research is unethical or not.
Cloning is a process by which genetically equal organisms are created with the same DNA. In simplest terms, clones are like twins born at different times. This procedure poses various dangers to society and humankind. One of the greatest threats this procedure creates is among
1) Robertson, John A. “Human Cloning and the Challenge of Regulation,” The New England Journal of Medicine, vol. 339, no. 2 (July 9, 1998), pp. 119-122.
What are the principle, ethical issues and experimental procedures used in genetic engineering and cloning? Should Cloning be allowed to continue?
Advances in modern medical science in the near future are dependent upon the advances in methods and procedures that, by today’s standards, are considered to be taboo and dangerous. These methods will not only revolutionize the field of medicine, but they will be the forerunners to a whole new way of treating people. For these advances to take place, several key steps need to be taken both medically and politically. In this paper I will attempt to explain what methods and procedures will be the future of modern medicine, how these methods and procedures can benefit mankind, and finally what changes will be needed in the fields of medicine and politics. First, I’ll attempt to explain which methods and procedures will be the future of modern medicine.