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Biology experiment exocyitment
Animal experiments
Animal experiments
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This experiment that was executed was about breeding fruit flies (Drosophila). The purpose of this experiment was to further understand autosomal and sex linked traits. The reason that fruit flies were used was due to the minimal cost, the minimal space they take up, their simple living requirements, and their short lifespan. We crossed females with wings and sepia eyes, which was a mutation, and apterous males with red eyes. The absence of wings is also a mutation. The question of the experiment was whether these mutations were because of autosomal inheritance or sex-linked inheritance. The hypothesis was that neither trait was sex linked. It was believed that the sepia eyes and the absence of wings were autosomal recessive traits and that red …show more content…
The hypothesis stated that neither trait was sex-linked and that the sepia eyes and the absence of wings were autosomal recessive traits and that red eyes and the presence of wings were autosomal dominant traits. According to the data, it can be concluded that this hypothesis was incorrect. However, both the group data and the class data were found to be flawed, and the hypothesis was actually correct. The results should have shown that these traits were autosomal and that the ratio of the phenotypes should have been 9:3:3:1. However, the class total ratio was 30:5.5:7.4:1 and the group total ratio was 39:4:10:1. One of the key observations that were made is that all of the F1 flies were red-eyed and had wings. This is because the parent flies that were put into the vial were homozygous for both traits. This caused the F1 flies to be heterozygous for both traits, and it caused the F2 flies to show all four phenotypes. The main major error that was made during the lab was the data. The data was wrong and should have been different. This had a major impact on our result. As per the statistical analysis, the hypothesis created was incorrect, but the data was so far off, that our hypothesis was deemed incorrect when it is in
Variation in selection pressures on the goldenrod gall fly and the competitive interactions of its natural enemies
Test 4: All three phenotypic frequencies saw a reduction in their number as the homozygote fishes saw a reduction in their number and were not able to pass on their alleles to create either their colored fish or a heterozygote. Both yellow and blue allele frequencies decreased by the same
Both some of the original flies and some offspring died during the experiment which could have influenced the results. If a vestigial fly died before it could mate that would give the wild type males an advantage in mating and producing a greater number of offspring. This would also be the case if any flies were harmed at any time during the mating period. To try and combat this problem the next time this experiment is done a larger number of male fruit flies could be used or more samples taken to try and reduce extremes in the data. Studying fruit fly mating behavior is very important because their generation length is so short and reproduction happens rapidly.
An individual can be homozygous dominant (two dominant alleles, AA), homozygous recessive (two recessive alleles, aa), or heterozygous (one dominant and one recessive allele, Aa). There were two particular crosses that took place in this experiment. The first cross-performed was Ebony Bodies versus Vestigle Wings, where Long wings are dominant over short wings and normal bodies are dominant over black bodies. The other cross that was performed was White versus Wild where red eyes in fruit flies are dominant over white eyes. The purpose of the first experiment, Ebony vs. Vestigle was to see how many of the offspring had normal bodies and normal wings, normal bodies and vestigle wings, ebony bodies and normal wings, and ebony body and vestigle wings.
For the second experiment, the reproduction of fruit fly was manipulated by placing different levels of ethanol into the test tubes in which adult males and females were inserted. First, 2 adult males and 3 adult females were placed inside test tubes for two weeks. These test tubes contained different levels of ethanol in order to determine how many adult flies would hatch depending on the concentration of ethanol in the environment. There were a total of ten groups containing different amounts of ethanol. The five groups of two contained 0%, 1%, 2%, 4%, or 8% ethanol. After the two weeks, hatched adult flies were anesthetized with Fly Nap in order to remove them from the test tube to calculate how many were produced in the different levels of ethanol. They were then counted to determine which level of ethanol concentration best allowed the fruit fly to hatch. After the counting of the flies the adult flies were disposed of into a jar of
The exercise involved a series of ‘mating’ events resulting in 6 generations. Each mating event produced offspring with ‘possible’ newly inherited traits. The idea of ‘chance’ was included through simple coin tosses. Also, ideas of selection and mutations were introduced into the ‘gene pool’, which presented a deeper and more clear understanding of Mendelian inheritance and the Hardy-Weinberg equilibrium. Upon reaching the third generation, A B1 mutant allele was introduced to the blue locus-influencing fin shape and a G1 mutant allele was introduced to the green locus-influencing Mouth
Albinism is a genetic condition present at birth, characterized by a small amount of melanin pigment in the skin, hair and eye. Albinism is an occasional inborn sickness related with vision difficult, which affect one in seventeen thousand persons. It is not a contagious disease and cannot be spread over contact. Albinism affects individuals from all races. Most folks with albinism have parents with a normal color of skin. Some may not even recognize that they are Albino until later on in their life. This paper will be based on the study of albinism, causes, types, the genetic transmission and some possible medical problem.
The white (w) eye color gene is located on the X chromosome at 1.5 genetic map units (1). The mutation is also recessive, meaning that each fly has different copies of the gene if they are either male or female (2). In wild-type Drosophila, the brick red color is visible due to the combination of two pigments, brown and scarlet. The synthesis of drosopterin for bright red pigments is controlled by the (bw+) gene and the synthesis of ommochromes for brown pigments is controlled by the (st+) gene (7). Therefore, there are two pigment synthesis pathways that must be working in order for the flies to express the brick red eye color. In addition, transport proteins are responsible for transporting both pigments into the eye in order to express the color (8). Thus, both the pathways responsible for the synthesis of brown and red pigments must work properly as well as the genes that encode for transport proteins. Despite having white eyes, Drosophila flies with this mutation still experience normal eyesight
In this experiment, Mendelain Models are observed. The purpose of the experiment is to understand how traits are passed from one generation to the other as well as understanding the difference between sex linked and autosomal genes. One particular trait that is observed in this experiment is when a fly is lacking wings, also known as an apterous mutation. In this experiment, we will determine whether this mutation is carried on an autosomal chromosome or on a sex chromosome. The data for this experiment will be determined statistically with the aid of a chi-square. If the trait is autosomal, then it will be able to be passed to the next generation on an autosomal chromosome, meaning that there should be an equal amount of male and
In my third year at Michigan State, I was enrolled in a class called Research in Biology. The goal of this course was to determine if there was a genetic marker to tell three different species of Rhagoletis flies apart due to their shared phenotypes and the infestation of apples, which became quarantined when one species was found in the orchards. If the other two species were found in the orchards, they would do no harm and the apples would be safe. Using their mitochondrial genomes, we ran gel electrophoresis and Nanodrop analysis and sent the DNA to Michigan State’s genomic core lab to be processed by Illumina. After getting the data back, our lab used a development node called Trimmomatic to eliminate adapter sequences, poor quality control bases, and ambiguous bases.
middle of paper ... ... avour of "purity of the race" idea, but they understand how it worked. Blond hair and blue eyes are recessive genes. Two brown-eyed people can give birth to a blue-eyed child, but two blue-eyed people cannot give birth to a brown-eyed child. Dark skin and dark hair are also dominant genes, so because of evolution, it must mean that the ancestors of humanity had dominant genes.
The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had to have been within the ratios of 9:3:3:1. The results were clear and allowed the null hypothesis to be correct. The white eyed gene in the fruit flies is sex linked. Sepia eyes and vestigial wings are not sex linked and are examples of independent assortment.
Both graphs and data tables show that no anomalous results were present. This is evident within the data as no one point cause a major shift in the trend of the results.
more than half the variation was found to be due to heredity. Among these traits were
...ary part in genotypes of potential interest that human geneticists breeders, as well as evolutionary geneticists are investigating. However, although we have the capability to unravel experiments that the founders of quantitative genetics would have never imagined, but their basic, un-computational machinery that they developed is most easily adaptable to the latest analyses that will be needed. We are far from ‘letting-go’ molecular biologists from the mathematical techniques/systems, because this age in respect to genomics has been forced into accepting gratitude due to the major importance of quantitative methods as opposed to the new molecular genetics. As geneticists tend to map molecular variation as well as genomic data, quantitative genetics will be moving to the front position because of its relevance in this age of rapid advancement in molecular genetics.