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Fly Thorax Cell Homogenate Lab Report

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The purpose of this particular experiment was to conclude which part of the fly thorax cell homogenate carries out glycolysis and which part carries out respiration. Methylene blue was the only true indicator as to whether glycolysis and respiration occurred. If dissolved oxygen levels were low then the methylene blue would become colorless and bleach. This was the case for four out of the seven test tubes. The results have shown that test tubes one, four, five, and seven did bleach. This is because the process of respiration had occurred in those tubes; therefore, the dissolved oxygen levels were low and bleaching was forced to occur. Test tube one bleached because it contained glucose and the whole homogenate which includes both the cytoplasm and mitochondria. Glucose was used in the cytoplasm to fuel glycolysis to eventually lead to respiration. Test tube four had glucose and the supernatant which fueled glycolysis and lead to respiration causing the tube to bleach. Test tube five had succinate and the pellet, which means it was lead straight into the kreb’s cycle eventually making the tube bleach. Test tube seven is unique on the reason it bleached because glucose and succinate were already present from the broken up cells; therefore, glycolysis was occurring before the…show more content…
Different reasoning led to different amounts of time in which they took to bleach. Timing ranged from ten to twenty minutes. Tube seven took the longest time of twenty minutes because its constituents were of the original and older broken up cells. These older cells still possessed the quantity; however, they did not possess the new and fresher quality hence the bleaching process occurred at a slower rate. Tube five took the shortest time of ten minutes because it contained succinate which is an intermediate for the kreb’s cycle; therefore, respiration was able to occur much faster than in the other
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