Finding out How the Concentration of the Starch Affects the Activity of Amylase Prediction I predict that as I increase the substrate concentration the activity of the enzymes will increase until a point where the rate of reaction will stay constant. My predicted graph will look like this The enzyme, busily going about its catalytic work, will simply hang around until a substrate molecule collides with it appropriately. In the same way that increasing the temperature increases the chances of a favourable collision, so increasing the substrate concentration increases the chances - because there are simply more substrate molecules kicking about! [IMAGE] However, there's only so much work that each enzyme molecule can do. Eventually you’ll reach a stage where there are more substrate molecules than there are enzyme molecules to work their magic - so, as the image hopefully shows, you've got too many substrate molecules for any extra to make any difference. If you raise the concentration any further, it will make no difference because there are no extra enzyme molecules to meet this supply. Background Knowledge Enzymes are chemicals which speed up (catalyse) other reactions. They do not actually take part in the reaction or change it in any way except to make it happen faster. Each type of enzyme acts on a specific type of food · Amylase breaks down complicated carbohydrate such as starch into simple sugar such as glucose · Protease enzymes break down the protein in food into amino acid Enzymes work best under very specific conditions of temperature and pH. Like most other chemical reactions, the ra... ... middle of paper ... ... I will be able to record it. During the whole experiment, we used only one measuring cylinder and we re-used it after cleaning it. We would have a new measuring cylinder for each experiment; therefore the experiment will be fair, as no minute amount of water will be left in the cornical flask. The result, aren’t as reliable as we wished If I had more time, I would repeat the experiment 3 times. Conclusion In conclusion, I feel that this experiment went successful, as it did everything as it answered the question ‘How does the concentration of the substrate affect the rate of reaction between Amylase and Starch Solution’. I can answer that by showing them the two graphs as well as the result tables, and by saying As the concentration of substrate increase, so does the rate of reaction.