Fermentation Broth Analysis: Analysis Of Chemistry In Chemistry

Objective: The purpose of this experiment was to produce aspirin using salicylic acid and acetic acid. Experimental Section: There were four parts to this lab. The first experiment, was synthesizing. A 600 ml beaker was filled with water and placed on the hot plate until it boiled. The measurement was .995g.

Protein quantification Five tubes labeled “Blank”, “C”, “LSS”, “HSS”, “HSP” were obtained. The blank contained 100 ul of distilled water. The others had 90 ul of distilled water and 10 ul of the cellular fractions obtained by centrifugation. #ml of BCA were added. The samples were placed in a water bath and incubated at 37 degrees Celsius for 30 min.

(SDS denatures the proteins, BPB is used as a tracking dye while Beta Mercaptoethanol breaks sulfide bonds.)  Heat the samples at 80 degrees Celsius for 5 minutes in a water bath. This helps in protein denaturation.  Put the cassette into the buffer dam and fill it with the running buffer. For SDS-PAGE, running buffer is prepared by taking 40 ml Tris-Glycine and adding 4 ml of 10% SDS to it.

This was to see the difference between the initial temperature and the final temperature. First Test In a 250ml beaker place 100mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved. After 1 minute measure the temperature and record it, do this for a further 2 minutes (3 minutes in total). Repeat this process for a total of 10 teaspoons.

The working standard solutions were prepared prior to use of stock solution by serial dilution method with mobile phase to yield a final concentration of 500ng/ml, 750ng/ml, 1µg/ml, 1.5µg/ml and 2µg/ml. Plasma Sample preparation A 2ml micro-centrifuge tube coated with EDTA was taken. 0.5ml Blood was taken and centrifuged at 1600 rpm for 10min at room temperature to separate plasma from blood. Now plasma was collected and stored at -20°C until use Plasma Extraction Procedure: A 200µl plasma sample was taken and thawed at room temperature. Now a drug solution of 10µl to 400µl was added as per requirement and vortexed for 3min.

To this, 0.1 ml ammonia solution was added, shaken thoroughly and centrifuged at 3000 rpm for 10 min. The alcoholic layer was separated and the colour intensity was read at 465 nm against amyl alcohol blank after 15 min. Sodium phytate standards were run along with the sample. The results were expressed as mg/100 g of

The agitation rate was increased from 200 to 500 rpm to maintain the level of dissolved O2 at 40 % saturation and the pH was adjusted to 7.0 by using 3 M NaOH. Crude palm kernel oil (CPKO) supplied by Acidchem Int. Ltd. was fed as sole carbon source for P(3HB) production at a maximum concentration of 46 g/L. After 48 h of cultivation, cells were harvested by centrifugation (9370 × g for 5 min) at 4° C. The pellets were then frozen at – 20 °C for 24 h before freeze-drying at 40 oC for three days. Biological Recovery of PHA Test Animal... ... middle of paper ... ...e determined from the DSC endotherm.

Three samples were analyzed for each time interval. After the required time, 1 ml aliquots taken were transferred to a 10 mL volumetric flask and neutralized with 1 mL 0.1 M NaOH using pH meter. This solution was diluted with mobile phase to 20µg/ml of FVS solution for the HPLC analysis. The kinetic determinations were performed in the dark to exclude the possible degradation effect of light. 2.5.2 Kinetic investigation of FVS in oxidative degradation The kinetics of the acid degradation of FVS were evaluated in 3 % H2O2 at 70°C for different time periods.

(1999). Freeze-dried avocado mesocarp (0.05 to 0.10 g) was mixed with 10 mL 80% (v/v) ethanol and homogenized for 1 minute. Thereafter, the mixture was incubated in an 80oC water bath for 60 min to extract the soluble sugars. Subsequently the mixture was kept at 4 ºC overnight. After centrifugation at 12000 g for 15 min The mass of each fruit was weighed before cold storage using a weighing scale.

Collect one 20 ml sample. Repeat with 90:10 hexane and collect 4 20-mL bottles. Repeat with 80:20 hexane and collect 2 20-mL samples. Analyze each fraction by spotting 10 times with capillary tubes on a TLC plate, which is exposed to iodine vapor for 15 minutes. III.