Experiment Determination Of Aspirin

Then add 0.8g NaOH to the cold Alclorite solution. Cool the NaOH/Alclorite solution to -10°C in the freezer. Grind 1.5 g phthalimide to a fine powder. Now add this to the cold (-10°C) Alclorite solution, plug the flask and mix with magnetic stirrer (or shake). The phthalimide will dissolve within 5 minutes.

This was to see the difference between the initial temperature and the final temperature. First Test In a 250ml beaker place 100mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved. After 1 minute measure the temperature and record it, do this for a further 2 minutes (3 minutes in total). Repeat this process for a total of 10 teaspoons.

Characterization of Aspirin. In the first section, the Synthesis of Aspirin, salicylic acid was weight to be 3.029 grams using mass by difference since it was weighed on a 150 milliliter beaker. 9.23 milliliters of the acetic anhydride and 14 drops of 85 percent phosphoric acid were added to this beaker. A Bunsen burner provided by the laboratory was then used to boil the just mixed combination by producing a flame underneath the positioned beaker on top, and then allowed to cool for several minutes after the Bunsen burner flame was terminated. Two quantities of distilled water were then added to this mixture to make it cool even further, which were 41 drops and 30 milliliters.

Then add two drops of iodine reagent to each of these wells and wait one minute, then add 0.5ml of amylase to each of the appropriate tubes. After two minutes, add a few drops from each tube, and place in the corresponding wall. After collecting the data use a color coding scheme to convert the qualitative data to quantitative data. The numerical data from each group was used to calculate the mean starch concentration and the standard deviation. The mean and the standard deviation values were calculated after entering all the data into

Protein quantification Five tubes labeled “Blank”, “C”, “LSS”, “HSS”, “HSP” were obtained. The blank contained 100 ul of distilled water. The others had 90 ul of distilled water and 10 ul of the cellular fractions obtained by centrifugation. #ml of BCA were added. The samples were placed in a water bath and incubated at 37 degrees Celsius for 30 min.

Then 25.0ml of hexane was added and the mixture was shaken for 5 minutes with occasional venting. The aqueous layer was discarded and the organic layer was left inside. About 25.0ml of 10% NaOH was then added and the mixture was shaken as before. The aqueous layer was collected and then cooled in an ice bath. It was then acidified with enough 6.00 M HCl while the pH is being monitored with red litmus paper.

(1999). Freeze-dried avocado mesocarp (0.05 to 0.10 g) was mixed with 10 mL 80% (v/v) ethanol and homogenized for 1 minute. Thereafter, the mixture was incubated in an 80oC water bath for 60 min to extract the soluble sugars. Subsequently the mixture was kept at 4 ºC overnight. After centrifugation at 12000 g for 15 min at 4 ºC, the supernatant was filtered through glass wool and taken to dryness in a vacuum concentrator.

(SDS denatures the proteins, BPB is used as a tracking dye while Beta Mercaptoethanol breaks sulfide bonds.)  Heat the samples at 80 degrees Celsius for 5 minutes in a water bath. This helps in protein denaturation.  Put the cassette into the buffer dam and fill it with the running buffer. For SDS-PAGE, running buffer is prepared by taking 40 ml Tris-Glycine and adding 4 ml of 10% SDS to it.

Next, for the first experimental setup, five (5) cups of tap water was poured in the casserole. Then, one (1) tablespoon of rock salt was dissolved in the tap water. The solution was boiled using the stove. The time (in seconds) when the solution boiled was recorded. Again, the procedure of boiling the tap water with one (1) tablespoon of rock salt was repeated for two (2) more times.

Small aliquots of 3g potassium dichromate were then added. Heating was resumed and when there was no more visible reaction, 3.5g of potassium oxalate was added. The beaker was then cooled in ice for about 8 minutes. 4cm3 of ethanol was added and the beaker was swirled. Vacuum filtration was carried out and the solid product was washed in an 8cm3 solution of 50:50 ethanol and water.