Evaluation of Fluensulfone for the Control of the Potato Cyst Nematode, Globodera Pallida

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Introduction
The potato cyst nematodes (PCN), Globodera rostochiensis (Woll) and G. pallida (Behrens), are the most economically important and well-studied cyst-forming species within the genus Globodera. Even though both species are well established within the main potato-growing areas of the UK (Gatwick, 1992; Trudgill, 1986), there has been an increasing incidence of G. pallida in England and Wales with 67% of infested fields, as of 1998, comprising purely of G. pallida (Minnis et al., 2002). This increase in G. pallida is suggested to be a pitfall of increased cropping frequency of potato carrying the H1 gene, expressing complete resistance to G. rostochiensis, but is susceptible to G. pallida (Trudgill et al., 1996).
Soil treatment with synthetic nematicides is commonly practiced in the UK (Whitehead, 1998; Whitehead, 1986) and the method has remained a key component of the integrated management of PCN (Haydock et al., 2006). Currently, granular forms of the organophosphate based fosthiazate (as Nemathorin 10G; Syngenta Crop Protection Ltd., Cambridge, UK) and the carbamate based oxamyl (as Vydate 10G; DuPont Crop protection Ltd. Stevenage, UK) are widely applied to manage PCN. Both nematicides are acetylcholinesterase inhibitors () and as such cause paralysis in nematodes (Opperman and Chang, 1990) consequently interfering with nematode activities such as hatching, mobility, host seeking, root infection, feeding, and reproduction (Woods et al., 1999; Hague and Gowen, 1987; Evans and Wright, 1982; Steel, 1982; Greco and Thomason, 1980). A single application of either product at planting has been shown to minimise damage to the crop and reduced population increase (reference). Available records on nematicide usage in Britain showed that potato growing land treated with fosthiazate and oxamyl has increased significantly over the decade leading up to 2010 (https://secure.fera.defra.gov.uk/) and could indicate an increased reliance on these products by growers for improving economic gross margin. Despite the impacts upon the current potato production system, changing EU legislation to safeguard the use of crop protection products (Hillocks, 2012; Anon, 2009) might restrict future availability of these nematicides. And if these substances become unavailable, the Potato Council estimated a two-fold increase in the economic cost of PCN (£26m per annum) (Twinning et al., 2009; Clayton et al., 2008). Besides, the increasing incidence of G. pallida in land treated with these nematicides provides evidence, which would support the suggestion of these substances not being very effective in controlling this species (Whitehead et al., 1994; Evans, 1993; Whitehead, 1992), and that soil treatments might have selected G.

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