Epinephrine And Forskolin Case Study

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Epinephrine and forskolin effect on the proliferation of mouse fibroblast cells.
Cell culture techniques allow the investigation of cells under different conditions and chemicals for a number of applications: to understand the metabolic activity of the cell, for large scale synthesis of biologicals such as proteins and to test out chemical compounds on specific cell type (Davis, 2011). Therefore the cell culture technique was used throughout this investigation to understand the effect of epinephrine and forskolin on the growth and cell structure of Ltk-cells. Epinephrine is an amino acid Tyrosine derived hormone secreted from the adrenal medulla glands in mammals under the stimulation of the sympathetic preganglionic fibres that can be caused by stress (Rayner and Dean, 2015). This promotes changes in the cell activity as epinephrine binds to the adrenergic receptor proteins within the target
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First observation took into account both adherent and non-adherent cells resulting in a higher cell confluency for all wells. On the other hand the second observation, the wells had the addition of crystal violet stain. Crystal violet stain increases visibility of cells under the microscope as the intensity of the staining is proportional to the number of adherent cells. Consequently a lower cell confluency was recorded for the second observation as the procedure of washing the cells with phosphate buffer saline and staining removes non-adherent cells (Table 1) (Coventry University, 2015). Furthermore, phosphate buffer saline was also used as the control. Due to its neutral pH, non-cytotoxic and isotonic behaviour (Thermofisher, 2015) it is ideal for comparison with other changing variables in the investigation. Moreover, further examination of the adherent cells displayed all cells acquired a circular polygonal shape distributed in clusters with various cells separated from the
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