Enzymes Experiment

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Lab 5. Enzymes PART 1. The Introduction. Question #1: How does the concentration of substrate affect the reaction rate? 1)Hypothesis: An increase in the amount of substrate will increase the reaction rate to an extent. 2)Supporting Argument: An enzyme is a catalyst, in this case a protein, that increases the rate of a reaction without being used up. A substrate is the area of a reactant that an enzyme attaches to in order to be modified into the product of the reaction. Once a substrate is converted, it is released and the enzyme can take up another substrate. Therefore, if there is more substrate available to an enzyme more product can be created (Campbell “Biology” 6th ed. text, pp. 96-99). 3)Experimental strategy: How I would test my hypothesis experimentally would be to have several test tubes with the same concentration of enzyme, but different concentrations of substrate. After a fixed period of time, each test tube would be measured for the amount of product produced. I would compare the amount of time that passed with the amount of product to determine the rate of reacti...

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