Electrophoresis

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Introduction In this classic experimentation differing variables that affect the electrophoretic mobility of proteins are tested in order to determine which is more effective in increasing the rate at which this technique is completed as well as if the concentrated applications of these variables will lead to improvements of this technique in the future. In order to obtain the answer to this inquiry, a well-thought out and formulated procedure will be implemented to produce results that are responsive to the independent variables outlined. In alignment with the procedure, it is equally necessary to ensure that the experiment is replicated in various trials to therefore validate the results received. Throughout this experiment, there are several trials in which two rival variables are put into play, the size of the sample and the pH of the buffer, in order to determine which is more effective in increasing a protein’s electrophoretic mobility, with hopes to produce data that will further the advancement of electrophoresis. The research gathered from this experimentation will be able to bring into focus the fallacies of using certain variables to increase protein migration and determine which variable is more effective in regards to the type of electrophoresis it is being applied towards. Instrumentation and Variables The materials used in this experiment consist of the following: 1. Electrophoresis chamber (BRL Life Technologies, Inc, Bethesda, MD, USA) 2. Electrophoresis power supply 3. Agar gel 4. Gel comb 5. Buffer(s) 6. Scientific stains to use as samples 7. Sample-loading device 8. Masking tape, if needed to seal gel form 9. HeLa cell line (where the protein will be extracted from) 10. Pipettes and Tips 11. Gloves 12. pH m... ... middle of paper ... ...u/classes/phys/phys461/phys450/ANJUM05/ This article defined that polypeptides are components of a protein as well as the connections between RNA and DNA. This article added to prior knowledge on how DNA, RNA, and proteins are connected and the reasons as to why they are analyzed during gel electrophoresis. Upcroft, P., & Upcroft, J. A. (1993). Comparison of properties of agarose for electrophoresis. Journal of Chromatography, 618 This abstract provided information on both the advantages and disadvantages of the utilization of agarose gel electrophoresis. This short summary served as the introductory paragraph for the information that is provided by the conductors of this experiment. The authors of this abstract gave insight on the shortcomings of agarose gel electrophoresis and thus enabled the development of inferences that will help throughout experimentation.

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