"Induction of Cytotoxic T Lymphocytes and Antitumor Immunity with DNA Vaccines Expressing Single T Cell Epitopes," by Frank Ciernik, Jay A. Berzofsky, and David P. Carbone explores the uses of the gene gun and how it can induce both humoral and cellular immunity. The paper specifically explores the effects of p53, a tumor repressor which gets its name from its molecular weight( p53 is a protein that has a molecular weight of 53). It is tremendously important because fifty percent of known cancer types stem from a mutation in this gene. A Brief History of Immunization Vaccines came about some 200 years ago when Jenner discovered that if someone caught a mild case of cowpox they would not get smallpox. In 1879, another scientist, Louis Pasteur, accidentally discovered the vaccine for fowl cholera by leaving cultures out in his laboratory. Later, Pasteur went on to develop an effective vaccine for rabies. The typhoid and cholera vaccines were produced by Wilhelm Kolle in 1896. The groundwork for tetanus and diptheria toxid vaccines was laid by Emil von Behring and Emile Roux in the early 20th century. In 1955, the polio vaccine, developed by Jonas Salk, was licensed. The Contagious and Non-Contagious Infectious Diseases Sourcebook estimates that a vaccine for chickenpox developed by Merrick Sharp Dohme will soon be available. ADVANTAGES There are many advantages to using gene immunization rather than protein immunization. For example, it is more effective at inducing cellular and humoral responses than protein. More importantly, it is safer. By targeting only the desired epitope, this method of immunization avoids the induction of unwanted responses. A current example of an unwelcome response would be... ... middle of paper ... ... tumor cells in the mice. Modern Applications of Genetic Immunization The implications of the proposed vaccines introduced by this research could be immense. Along with the advantages over traditional vaccines, they may be applicable to infectious diseases of which no preventative measures are currently known. Effective immunization for infectious diseases could include innoculation from: BSE/ Cholera, Dengue, Ebola virus, Hantavirus pulmonary syndrome, Hepatitis B, C viruses; Herpes simplex virus, HIV, Influenza, Malaria, Meningitis-causing enteroviruses, Papilloma virus, Rabies virus, Tuberculosis, and Yellow Fever. In addition, DNA epitope vaccines may elicit protective immune responses against cancer. Induced response against identified T cell epitopes including the inhibition of tumor growth could be the result of this break-through technology.
The article’s information is presented with the goal of informing a reader on vaccines. The evidence is statistical and unbiased, showing data on both side effects and disease prevention, providing rates of death and serious illness from both sides. This evidence is sourced from a variety of medical organizations and seems reliable, logical, and easily understood, no language that would inspire an emotional response is used. The validity of studies is not mentioned in the article, but it does encourage readers to investigate further to help make a decision. The article allows a reader to analyze the presented evidence and come to their own
Louis Pasteur could easily be considered one of the greatest patrons of humankind his work in the discovery of vaccinations for rabies, anthrax, chicken cholera and silkworm diseases contributed greatly to society (Rhee, 2014). Pasteur’s accomplishments point to singular brilliance and determination of Pasteur's nature. His work aided in developing medicines in areas such as stereochemistry, microbiology, bacteriology, virology, immunology, and molecular biology. Furthermore, his work has safeguarded millions of people from disease through vaccination and pasteurization (Rhee, 2014).
Each day researchers are finding out about vaccines and are realizing that there are a lot more risks than benefits. Dr Phillip F. Incao explains: “Today, far more children suffer from allergies and other chronic immune system disorders than from life-threatening infectious disease. It is neither reasonable nor prudent to persist in presuming that the benefits of any vaccination outweigh its risk” (qtd in Spaker). While infectious diseases are becoming uncommon there is no need for any person to get vaccinated. There have been many issues surrounding vaccinations all around the world.
Vaccines have been used to prevent diseases for centuries, and have saved countless lives of children and adults. The smallpox vaccine was invented as early as 1796, and since then the use of vaccines has continued to protect us from countless life threatening diseases such as polio, measles, and pertussis. The Center for Disease Control and Prevention (2010) assures that vaccines are extensively tested by scientist to make sure they are effective and safe, and must receive the approval of the Food and Drug Administration before being used. “Perhaps the greatest success story in public health is the reduction of infectious diseases due to the use of vaccines” (CDC, 2010). Routine immunization has eliminated smallpox from the globe and led to the near removal of wild polio virus. Vaccines have reduced some preventable infectious diseases to an all-time low, and now few people experience the devastating effects of measles, pertussis, and other illnesses.
A time where vaccines were beneficial was when small poxs found its way around the human population. It was only till the end of the 18th century where an effective vaccination created by Edward Jenner was made for
Edward Jenner invented a method to protect against smallpox in the late 1700s. The method involved taking substances from an open wound of someone with small-pox or cow-pox and injecting it into another person’s skin, also called “arm-to-arm inoculation”. The earliest actual documented examples of vaccination date all the way back to the tenth century in China (Lombard, “A brief history of vaccines and vaccinations”). The mention of early vaccination was taken note of by a French scholar, Henri Husson, written in one of his journals (Dictionaire des sciences médicale). The Ottoman Empire Turks also discovered a method of immunization a few centuries later. Lady Montagu of Great Britain, a famous writer and wife of the English ambassador of Istanbul, between 1716 -1718, came across the Turkish vaccine for small-pox. After surviving as a child with small-pox, she insisted her son be vaccinated (Henricy, “Letters of the Right Honourable Lady Wortley Montagu”). When she returned to England, she continued to publicize the Turkish tradition of immunization and spread their methods to the rest of her country. She also had all family members also vaccinated. Immunization was soon adopted in England, nearly 50 years before Jenner's smallpox vaccine in 1796 (Sharp, “Anti-vaccinationists past and present”). Edward Jenner’s target for smallpox was to eradicate it. And later by the 1940s, knowledge of the science behind vaccines had developed and soon reached the point where across-the-board vaccine production was a goal that was possible and where serious disease control efforts could start. Vaccines for many dangerous diseases, including ones protecting against pertussis, diphtheria, and tetanus were underway into production. ...
Edward Jenner is often regarded as the “Father of Immunology” for his development of the smallpox vaccine. His remarkable discovery has laid the foundation for future scientists working with immunizations. Jenner’s impact is seen worldwide to this day with the complete eradication of the deadly smallpox virus. Edward Jenner’s Legacy will always live on as the first to vaccinate using a live virus. Vaccines are improving everyday, which benefits the public’s health, all thanks to Edward Jenner.
The first discovery was made in 1952, in the developing field of virology. Virology is the study of viruses and how they behave. To develop the vaccines for the viruses, researchers infected the HeLa cells with many types of infections, such as measles, mumps, and the infamous poliomyelitis virus, also known as Polio. According to the Centers for Disease Control and Prevention (CDC), whose mission is to save lives and protect people’s health security, Polio is a "crippling and potentially deadly infectious disease caused by a virus that spreads from person to person invading the brain and spinal cord and causing paralysis" (Freeman). Jonas Salk, who was a virologist at the National Foundation for Infantile Paralysis (NFIP), used inactivated viruses (virus particles grown in culture and then killed by a form of heat) to create a polio vaccine. Salk drew blood from about two million children, which the NFIP checked for immunization.Through the collection of many HeLa cells and trial and error, the polio vaccine wa...
...gens are exogenous (outside the cell) and will be presented to helper T cells to initiate an immune response. This can trigger cytotoxic T cells to kill cancer cells with the same antigen – often HPV viral proteins in cervical cancer. T cells may not be activated to their full potential – recall that the inhibitory receptor CTLA-4 on T cells sends a stronger signal than CD28, the activating receptor. Ipilimumab is added to treatment for this reason. It will work in conjunction with the released antigens, activating the T cells that can respond to the antigens and create an immune response against the cancer cells (LACC article). Adding ipilimumab to the chemo/radiation treatment would enhance the immune system’s ability to respond to the antigen released by the treatment. This is the first time a treatment like this has been suggested for cervical cancer (LACC).
The history of differentiating between diseases and vaccinating them is a practice that has been used for more millennia than you can count on two fingers. In 900 BC, a Persian physician named Rhazes was the first to publish a written account attempting to distinguish between measles and smallpox (successful or not is a whole other matter). It takes about 2500 years before any more development in the field of vaccination. In 1661, Chinese Emperor Kiang wrote a letter that stated that he fully supports inoculation, which is the introduction of a pathogen or antigen into a living organism to stimulate the production of antibodies. Then in 1676, English doctor Thomas Sydenham publishes ‘Medical observations on the history and cure of acute diseases’ which successfully distinguishes measles from smallpox while in great detail. The report also stated details about Scarlet Fever which was big at the time. In 1678, a Boston newspaper published America’s first medical work, Thomas Thatcher’ pamphlet: A Brief Rule to Guide the Common People of New England how to order themselves and theirs in the Small Pocks, or Measles. Once again, Thomas Sydenham discovers a medical breakthrough in 1684 by concluding that the common health practices, not available to the poor, were more harmful than good in mild smallpox cases. Sydenham’s discovery would be the last big medical innovatio...
A remarkable breakthrough in medicine occurred in the late 1800s through the work of Louis Pasteur. Pasteur's experiments showed that bacteria reproduce like other living things and travel from place to place. Using the results of his findings, he developed pasteurization, which is the process of heating liquids to kill bacteria and prevent fermentation. He also produced an anthrax vaccine as well as a way to weaken the rabies virus. After studying Pasteur's work, Joseph Lister developed antisepsis, which is the process of killing disease-causing germs.
Lechner F, Jegerlehner A, Tissot AC, et al. Virus-like particles as a modular system for novel vaccines. Intervirology 2002; 45: 212-7.
For many years before the development of vaccines, it was known that after recovery from certain diseases some people would not become infected when exposed to it again. This course by which a person is protected from certain diseases after natural infection is termed active immunity. The person is protected since the immune system remembers the past infection and reacts quickly when it comes across the issue again. Yet, for diseases that can be life-threatening, attaining immunity in this way entails running the risk of death upon the first encounter. Even for non life-threatening diseases, a lot of infections carry a risk of grave complications after recovery and so it would be preferable to obtain immunity without taking unwarranted risks. Active immunity by way of vaccination presents a much safer alternative (Childhood Vaccinations: Understanding Vaccines, 2006).
The history of vaccine started with the spread of smallpox disease. Smallpox was a contagious disease and, it was spreading fast leaving permanent scars on patients' faces or worse taking their lives. At the time, there were several attempt to treat and prevent smallpox, but Edward Jenner had the greatest rule in eliminating smallpox.“Jenner's work represented the first scientific attempt to control an infectious disease by the deliberate use of vaccination”. ( “Conclusion” 1,2). Nowadays, Statistics show significant reduction in the cases of infectious diseases after the widespread of vaccination. There were annually 63,000 cases of Pneumococcal among children in the United States. After the beginning of vaccination, the cases redu...
For innumerable centuries, unrelenting strains of disease have ravaged society. From the polio epidemic in the twentieth century to the measles cases in the latter half of the century, such an adverse component of nature has taken the lives of many. In 1796, Edward Jenner discovered that exposure to cowpox could foster immunity against smallpox; through injecting the cowpox into another person’s arm, he founded the revolutionary concept known as a vaccination. While many attribute the eradication of various diseases to vaccines, many United States citizens are progressively beginning to oppose them. Many deludedly thought that Measles had been completely terminated throughout the United States; however, many children have been patronized by