EXTRACTION OF VITAMIN E FROM PITHECELLOBIUM JIRINGAN (JACK) PRAIN USING SOXHLET EXTRACTION

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ABSTRACT Pithecellobium Jiringan (Jack) Prain is one of the medicinal plant sources that consist of Vitamin E. The seeds have many benefits for health due to vitamin E content which can be used as anti-oxidant, anti-high blood pressure and anti-aging. This research was carried out to identify the best parameter in order to obtain the high quality of vitamin E. The Vitamin E was extracted from P.Jiringan seeds by using Soxhlet extraction method. The parameters involved in this research are extraction time (4 to 8 hours) and type of solvents used (hexane, methanol and water). In this research, the highest overall yield was obtained using methanol as a solvent with 31.04% for eight hours extraction time. In order to identify and quantify the vitamin E present in the extracted oil Ultraviolet-Visible Spectrometer (UV-VIS) was used. The result obtained indicated that hexane is the best solvent to extract Vitamin E which has the highest concentration, 93.165 ppm for eight hours extraction time. Besides that, Gas Chromatography Mass Spectrometry (GC-MS) also was used in order to identify and quantify the other interest chemical constituents present in the extracted oil such as medicinal compound, noxious compound and also essential oil. The antioxidant activity from vitamin E that extracted from P.Jiringan seeds will be examined by using DPPH method. The expected result that will be obtain from the analysis is 200, 000 trolox equivalent (TE) per 100g of sample. Keywords: P.Jiringan, Vitamin E, Soxhlet Extraction, GC-MS, UV-VIS Spectrophotometer, 2,2-diphenyl-1-picrylhydrazyl (DPPH) INTRODUCTION Pithecellobium Jiringan (Jack) Prain is one of the many medicinal plants which are totally beneficial for human health which contains t... ... middle of paper ... ... seeds Figure 7: Comparison amount of Eicosene in P.Jiringan seeds Figure 8: Comparison amount of 9,12-Octadecanoic acid methyl ester in P.Jiringan seeds CONCLUSIONS The extraction of Vitamin E from P.Jiringan seeds using Soxhlet Extraction was studied using two parameters which are solvent and time. From this study, the conclusion achieved is that hexane is the most suitable and effective solvent to extract Vitamin E from P.Jiringan seeds using Soxhlet Extraction, and that the longer the contacting time between solvent and the solute, the more yield and Vitamin E will be extracted. Meanwhile the antioxidant activity of vitamin E from P.Jiringan seeds can be determined accurately, conveniently, and rapidly using DPPH and the expected result that will be obtain from the analysis is 200, 000 trolox equivalent (TE) per 100g of sample.

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