Escherichia Coli, or E. Coli is a bacterium commonly found in the stomach of warm blooded organisms, animal feces, and on hot springs. Most strains of this bacterium aren’t harmful to humans. In fact, it has many beneficial jobs in our system including the production of vitamin K2, food digestion, and the prevention of harmful bacteria prospering in our intestines. O157:H7 is the only type of E. Coli that harms humans, often causing food poisoning and can be life threatening.
Structure:
E. Coli is a rod shaped bacteria which has adhesive fimbriae. It has only one chromosomal DNA and a plasmid. Some strains are capable of accepting and transferring plasmid to and from other bacteria which allows E. Coli to survive in stressful environments.
Living conditions:
This bacteria prefers to live in higher temperatures rather than cooler ones and thrive in a temperature of about 40 degrees Celsius. E. Coli can live with or without oxygen, and in prime conditions can reproduce in as little as 20 minutes. It grows best at a pH level of 7 and adapts well to our system because of the various and plenty nutrients.
Culture procedure:
The way we cultured the bacteria was with Agar which is a polymer made up of subunits of the sugar galactose. This is used instead of gelatin because it is fairly stronger and the bacteria won’t eat it. It is made by pouring the substance into boiling distilled water and letting it cool. We made sure everything was sanitary and poured the agar into our plates before allowing it to cool.
(Nordqvist, 2011, Medical News Today) (Laurie Usinger, 2012, Bio-Rad Laboratories)
Growth phases of bacteria:
Antimicrobial:
Listerine Total Care was put in four different plates with E. Coli. The active ingredient in t...
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...ted our hypothesis since the 100% concentration killed significantly more bacteria than the other concentrations. However, the 50% and 25% concentrations showed the opposite of what our hypothesis predicted. This doesn’t mean our hypothesis was wrong, but rather that we made human errors along the way. Some errors could have been the cleanliness of our equipment. If the previous group left residue of antimicrobials on the things we used, the bacteria was probably impacted by that. We also could have improperly spread the bacteria on the plates, giving some sides a higher concentration of bacteria than other sides. This would have also distorted our outcomes. Some things we could do to improve this experiment is by boiling every piece of equipment before using it and by figuring out a more efficient and equal way to distribute bacteria on the plates.
Bibliography:
Other human errors could have affected the results, such as not inverting the plate before putting it into incubation would not allow for bacterial growth. Not pipetting the tube up and down to mix the bacteria that settled at the bottom of the tube before starting the Gram Stain would not allow for an accurate reading because there wouldn’t be many bacteria on the slide. Passing the slide over the bunsen burner too many times, hence killing the bacteria and not allowing for a Gram Stain. If this experiment had to be redone, one improvement would be to allow for more than one plate without a point deduction. Unexpected human errors might interfere with a person’s results.
In this experiment the heat shock method will be used to deliver a vector (plasmid) of GFP to transform and grow E. coli bacteria. Four plates containing Luria Bertani (LB) broth and either –pGLO and +pGLO will have E. coli bacteria added to it. The plate containing –pGLO (no pGLO) and LB will show growth as ampicillin will be present killing bacteria but no glowing because no arabinose will be present for glowing to be activated, the same result will be seen in the plate containing +pGLO, LB and ampicillin. The plate with –pGLO, LB and ampicillin will show no growth and no glowing as no arabinose is present for glowing to be activated
First a control was established for E. coli in a 1.0x nutrient broth. This was
E. coli are bacteria that can cause an infection in various parts of your body, including your intestines. E. coli bacteria normally live in the intestines of people and animals. Most types of E. coli do not cause infections, but some produce a poison (toxin) that can cause diarrhea. Depending on the toxin, this can cause mild or severe diarrhea.
Scoffield, H. (2011, October 03). Ottawa to review safety of key ingredient in anti-bacterial soaps. The
E. Coli is a bacteria that is inside your intestines that helps you break down food. When cows are fed corn, it also decreases the healthy acids such as Omega-3 and increases Omega-6 which is unhealthy. We should also care about their living conditions because a cow is in one area for most of the day just eating corn and when cows eat corn they tend to poop a lot. It is crowded in the eating area for cows so when they poop it falls on the ground and they eat so much corn that they just keep pooping so eventually they start stepping in it and this would cause them to get diseases. Some farmers when they slaughter them do check for diseases but some don’t, so the diseases travel with the cow and onto your dinner plate which means that you could be eating an infected cow without knowing it.
We observed the agar plate for colonies that had a metallic look, because these were likely E.coli colonies. We then recorded what we saw. After selecting what we believed to be an E. coli coliform colony, we inoculated a TSA slant, in addition to another lactose broth, and incubated these for another 24 hours at 37°C.
E Coli is a pathogenic bacteria that can be in the intestine of warm blooded animals and humans. It can help break down food in the gut, and allows vitamin K to be absorbed. Its full name is Escherichia Coli, and although many strains are harmless, some can cause food poisoning in humans by making harmful chemicals.
Clorox and Lysol are the most commonly used disinfectants in the country. Disinfectants are used to kill bacteria which are microorganisms that grow rapidly and that can be found everywhere. Bacteria cannot be seen with the naked eyes but they are present, on your phone, desk, computer, even in the air, and they can also lead to diseases such as cholera. Some people prefer Clorox over Lysol to clean with. These two disinfectants had been tested to find out which one is the most effective at killing bacteria. Clorox is often said to be the best due to containing an active ingredient known as sodium hypochlorite which contains antibacterial activity. To test this hypothesis, a table was sprayed in three different sections, one with Clorox, one
E. Coli and other foodborne illnesses are something that should be of major concern to everybody, becasue nobody is safe from it. It is not something that can be prevented or
E. coli, short for Escherichia coli, normally live within the digestive system of humans and animals. Most variants of this bacteria are mostly benign and are actually essential for digestive health in people and animals. But there is the more infamous and most dangerous strain of E. coli, notorious for poisoning people and causing serious symptoms and even death. It has been labelled as E. coli O157:H7, discovered in 1982. This specific strain produces a toxin with the name Shiga that causes symptoms such as kidney failure, bloody diarrhea, and death. There have been outbreaks of O157:H7 in the US, Canada, Europe, South Africa, and Eastern Asia. These types of E. coli are labelled as pathogenic, meaning they can spread and cause illness outside
coli is spread from animals to people through by consumption of contaminated food and liquid. Common foods and liquids that might cause E. coli outbreaks include undercooked or raw hamburgers, salami, spinach, lettuce, sprouted seeds, unpasteurized milk, apple juice, and well water or water that animals frequently use. Other high-risk actions include failure to wash hands after contact with animals, and swimming in water that has low levels of sewage contamination (5). Enteropathogenic E. coli is seen all over the world but is more prominent in developing countries because of the lack of sanitation practices. It also is more frequent in younger people including children and infants less than two years of age. Therefore most of the E. coli outbreaks occur either in daycare centers or in pediatric wards.
There is also the potential of human error within this experiment for example finding the meniscus is important to get an accurate amount using the graduated pipettes and burettes. There is a possibility that at one point in the experiment a chemical was measured inaccurately affecting the results. To resolve this, the experiment should have been repeated three times.
Inconsistencies in this lab could have caused variations in data collecting. Collecting data from one petri dish was challenging because something could have been different on other petri dishes if this experiment was tested on several petri dishes. This could have been different because the other petri dishes could have had more micro-organisms in Section 2 instead of Section 1, or no bacteria could have grown at all in every section of the petri dish.- Second, nothing grew in section B even though there were no disinfectants in that section. The reason why the bacteria and mold might have grown in sections 1, 2, and 3 was because in the process of making the experiment, the coffee filter papers were touched with glove free hands and were not clean. If this lab was run again, some changes would be to wear rubber gloves, do not pour the hand sanitizers on the coffee filter paper but just pour one pump straight into the petri dish, have more than one petri dish to collect data off of, and check when the last time someone cleaned the door knob
Different types of bacteria have different range of temperature they are able to survive. They are generally divided into three types: psychrophiles, mesophiles and thermophiles. Psychrophilic bacteria are able to survive in low temperatures ranging from about -10 to 20°C while thermophilic bacteria are able to thrive in high temperatures ranging from 40 to 75°C. These two types of bacteria are also known as extremophiles due to their ability to survive in extreme conditions. Mesophilic bacteria are bacteria that thrive in temperatures ranging from 10 to 45°C and usually have an optimum growth temperature of about 37°C (M. Furlong, n.d.).