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Chapter 7—Experimental and Quasi-Experimental Designs
Essays on experimental designs
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The purpose of this experiment is to observe the preference of Drosophila Melanogaster between Coke and apple juice.
D. Melanogaster, the common fruit fly, is a small species of fly which feeds on fruit, yeast, alcohol, sap, and various plants. (Orkin) D. Melanogaster is a popular animal to use as a model organism--an organism commonly kept in the lab, which has been studied and has a lot of available information on it. Model organisms are called "model" because results of studies and experiences performed with them can often be generalized to humans or to other animals.
Because they can be easily cultured at a low cost, D. Melanogaster is an extremely popular model organism; their simple genetic makeup of only four chromosomes lends
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This suggests that, when all over variables are controlled, D. Melanogaster prefers a substance with a greater concentration of fructose--the apple juice. Since flies are also attracted to carbon dioxide, this plus the fructose and other sugars found in Coke are a greater attractant to D. Melanogaster.
These confusing results could have been avoided if the samples of apple juice and Coke were replaced with fresh samples for each trial. By introducing the flies to fresh Coke during each trial, we could see more clearly the flies' preferences. It is important to have as few variables as possible during an experiment; this experiment failed due to not considering the diffusion of carbon dioxide.
Conclusion
During the first trial of apple juice vs Coke, D. Melanogaster preferred the Coke; the flies preferred the juice in both subsequent trials. Because they were much more strongly attracted to the Coke during the first trial, before all the carbon dioxide had been released and diffused, the results seem to support the hypothesis that D. Melanogaster will prefer Coke over apple juice because of their attraction to carbon dioxide. However, the results are inconclusive--because the same sample of Coke was used in each trial, the experiment failed to control all the variables.
I hypothesized that sowbugs preferred damp soil, to dry, because moisture was the perceived causative agent. I observed that the sowbugs spent more time on the damp soil, rather than the dry soil (Table 1). The sowbugs spent 85% of their observed time on the damp soil side of the experimental chamber (Table 1). These results support my hypothesis, because they suggest that moisture of soil determines where sowbugs will prefer to spend their time (Table 1).
To conduct the experiment, the beetles were massed, then attached to a petri dish with a 30 centimeter piece of dental floss. The beetle’s mass was the independent variable. Afterwards, the floss was tied to the beetle’s midsection with a slip knot. Then, the beetle was placed on a piece of fabric with the petri dish attached to it. As soon as the beetle was able to move with one paperclip inside the petri dish, more were added, one by one, until it could not move any further. After the beetle could not pull any more, the paperclips were massed and the results were recorded. The dependent variable was the mass that the beetles could pull. No control group was included in this experiment.
The role of questions in Dunn’s article is to persuade the reader into thinking their own role or responsibility in the behavior of flies. The first question he poses is; “Just where do houseflies pick-up these other bacteria, the one they give back to us in vomit spot, feces and footsteps?” This question is central to his main idea. He poses a second question about the discovery made by his friend named Coby, on pig farms, asking; But why would the flies in pig farms tend to have antibiotic resistant bacteria? Yet again dragging the attention to the issue at hand that is that us humans are largely to blame for the dangers that flies poses to our
Planaria usually live in water, water doesn’t have any effect on them which the reason why it is used as a control group in this experiment. Planarian will be put near the edge in a two-chamber tray containing water, in the side that is not covered with a dark piece of paper. Five planaria will be tested in a 2- chamber tray. We will measure how long it takes the planaria to move from the edge of a chamber receiving light, to the dark chamber. Subsequently, start by adding the lower concentration of caffeine in the water, replacing planarian in the same position and measuring how long it takes now to go back to the dark chamber in the presence of caffeine. We will duplicate the same procedure with higher concentrations of caffeine and additionally take the average of the time all five planaria took to move from the edge of the chamber in brightness, to the dark chamber in different concentrations of
Introduction: The purpose of this laboratory activity is to investigate the Hardy-Weinberg Law of Equilibrium using the fruit fly Drosophila melanogaster. According to the Hardy-Weinberg Law of equilibrium, allele frequencies should remain the same in large populations that do not experience gene flow, mutations, nonrandom mating, and natural or artificial selection. We will be studying the alleles that determine wing shape, either normal (wild type) wings or vestigial wings.
Richard Conniff uses strong scientific facts in his essay to get his major point across to the audience. One of the major facts he uses stood out as it disproved many a wives tale about the common household fly. Most people think of flies as dirty animals who fed on dead animals and spread disease. Conniff uses a study that found flies tested from dirty areas contained more a lot mor...
We then allowed the larvae to hatch, and counted and recorded the total number of flies, the phenotype, and the sex. After taking down all this information this would allow us to perform a F1 cross, we made sure to examine the flies carefully since we needed virgin flies. We prepared a new vial with the a 1:1 ratio of medium and water. After recording the data of the F1 generation, and picking out the virgin flies for the crossing, and we killed of the rest of the flies using the oil method. After some time passed the F1 generation had larva in the vial. Once we noticed the larva we had to put the flies to sleep and collect the data. We then had to prepare another two new vials and medium and water. Carefully observing the flies and picking out three males and three female virgin flies to place into the new vial. Than killing of the other flies. After about a week we had the F2 generation. This was the most important generation, it was what we were looking for to allow us to observe and compare our experiment to Mendel’s experiment. We were looking for a 9:3:3:1 ratio with our flies. Using a basic Punnett square table and the crossing that we had accomplished our results should have looked like the following Punnett square.
My experiment is testing one of the five senses of my dog, Blaze. I am testing for taste. In this experiment the question I am going to answer is, Which of three flavors of treats will my dog prefer? The treats are made by the same company, Beggin’ Strips from Nestlé Purina PetCare Division, but they are different flavors. The flavors used will be Hickory Smoked Bacon, Bacon & Cheese, and Bacon & Peanut Butter. My hypothesis for this experiment is, Of the three flavors my dog will prefer the Bacon & Cheese flavor the best. I chose this topic for my experiment because I have a major interest in animals and I am hoping to get a degree in some form of veterinarian services, such as zoology.
As useful as their tongue is for collecting nectar it is useless in capturing insects hidden inside flowers, even though insects do provide most of the protein...
Daly and Wilson also refer to the research done by a British geneticist named A.J. Bateman to strengthen their arguments for the idea of status competition. Bateman's research focused on lab experiments done on Drosophila or fruit flies. The experiments consisted of taking fruit flies with "distinct genetic markers" and placing them in jars. It was made sure that each jar contained an equal number o...
The objective of this experiment is to determine what genes are responsible for the white-eye color in two strains of Drosophila melanogaster, known as the common fruit fly. Drosophila is used as the experimental organism for many reasons which include its small size, easy maintenance, short 10 day generation time, and a fully sequenced genome. The characteristics of the wild type, which is the most common phenotype found in nature, include brick red eyes, long wings, gray/tan body, and smooth bristles. Of course, there are mutations that occur that cause specific traits to deviate from the wild-type phenotype. These traits include wing length, bristle shape, body color, and eye color.
“Organic Wisdom, or Why Should a Fly Eat Its Mother from Inside.” Ever Since Darwin. New York, New York and London, England: W.W. Norton & Company, 1977.
The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had to have been within the ratios of 9:3:3:1. The results were clear and allowed the null hypothesis to be correct. The white eyed gene in the fruit flies is sex linked. Sepia eyes and vestigial wings are not sex linked and are examples of independent assortment.
The purpose of this lab was to study the response of the genus Daphnia to chemical stimuli and to examine human responses to different stimuli. A stimulus is an incentive; it is the cause of a physical response. Stimuli can have a physical or chemical change; an example of a physical change is a change in temperature and sound. An example of chemical change would be changes in hormone levels and pH levels. Muscular activity or glandular secretions are responses that occurs when stimulus information effects the nervous and/or hormone system. Daphnia is a genus; it is a small crustacean that lives in fresh water. The body of the daphnia is visible and its internal organs are clearly seen thus it was chosen for this exercise. The
Deilephila elpenor moths were trained to associate a sugar reward with a color, either blue or yellow by feeding from colored artificial flowers at a light intensity equivalent to late dusk.