Chemotaxis Case Study

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(ii) Motility and chemotaxis. In addition to their role in the attachment of microorganisms to plant roots, functional flagella are important for bacterial motility. The crucial role of motility for successful rhizosphere colonization is somewhat controversial because some studies have indicated that motility of Pseudomonas is not required for root colonization in wheat and soybean (Howie et al. 1987). However, flagella were shown to be essential for colonization of potato roots (de Weger et al. 1987). Studies confirming the role of motility in the colonization process were performed in the absence of percolating water, and it was assumed that motile or non-motile introduced bacterial strains were transported by the growing roots. However, under more natural conditions the presence of percolating water will affect the dispersal of bacterial strains regardless of their ability to swim. …show more content…

Chemotaxis plays a role in the establishment of both deleterious and beneficial plant-microbe associations, and experiments with mutants defective in the general chemotaxis gene cheA have revealed that in the absence of percolating water, chemotaxis is crucial for competitive colonization of tomato roots by P. fluorescens WCS365 (Dekkers et al. 1998a; Lugtenberg et al. 2001). In P. fluorescens rpoN mutant has reduced ability to colonize plant due to defect in numerous attributes including flagella and for absorption of wide range of nutrient sources including sugars, organic acid, inorganic nitrogen, stress tolerance and protein secretion for which RpoN is mandatory. RpoN-regulated genes contribute to acid tolerance and resistance to some antibiotics, including tetracyclines and aminoglycosides indicated by chemosensitivity assays (Jones et al.

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