The Effects of pH on Catalase Activity
Problem and introduction:
Catalase is an enzyme in living plants and animals that speeds up the breakdown of hydrogen peroxide (H2O2). The breakdown of hydrogen peroxide is essential to living organisms because hydrogen peroxide is toxic to cells. The chemical reaction for the decomposition of hydrogen peroxide is having the substrate hydrogen peroxide catalyzed by the enzyme catalase to produce the products, water and oxygen. What effect does pH have on the enzyme catalase and its ability to decompose H2O2?
Hypothesis:
As the pH gets further away from the pH of 7, the enzyme catalase will decompose more hydrogen peroxide and more bubbles will form.
Materials:
Catalase solution at 400 units/mL
10 test tubes
Distilled water
Hydrogen peroxide
Buffer pH 5
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Verify that the pH is approximately 3 or lower.
Add 1 mL of NaOH to tubes 8 and 10. Verify that the pH is approximately 11 or higher.
Add 1 mL of the buffer solutions as indicated in Table 3.
Your instructor may ask you to verify that the buffers produce the indicated pH. If so, use pH paper to measure the values for each solution and record them in Table 3.
No catalase is added to tubes 1, 3, 9, or 10.
Add catalase to tubes 2 and 4-8 according to Table 3.
Add hydrogen peroxide to tube 3. After adding hydrogen peroxide, swirl the solution gently and immediately record in Table 4 qualitative changes in the bubbling intensity of oxygen production on a scale of 0 (no bubbling) to 5 (vigorous bubbling).
Repeat step 11 for each remaining solution.
After you have gathered your data for all 10 tubes, record in Table 4 your explanation for the results of the catalase activity in each of the tubes.
Clean your work area and materials. Follow your instructor’s directions concerning the disposal of waste solutions containing HCl and NaOH.
Table 3: Experimental Conditions to Test the Effects of pH on Catalase Activity
Tube
Distilled Water
Buffer
H2O2
Of the pH values tested, which is the optimal pH? Use the results in Data Table 3 to support your answer. Hypothesize how the structure of the lactase relates to the results in Data Table
We then took 1ml of the 10% glucose solution again using the glucose rinsed pipette and added it to test tube 1, we then filled the H2O rinsed pipette with 9ml of H2O and added it to test tube one; making 10ml of 1% solution.
The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
To record the results of Oxygen created from decomposing Hydrogen peroxide with catalase that are either heated, cooled of left in room temperature.
The purpose of this assessment was to research, design and conduct an experimental investigation on the effect of substrate concentration (manipulated by increasing concentration of pH buffer) of catalysed reactions by measuring the volume of oxygen produced as the reaction proceeded.
How does the temperature (-2°C, 20°C, 30°C, 40°C, 60°C) affect the production of oxygen (cm3) from cow hepatic (the enzyme catalase) when placed in boiling tube with 10 ml of 3% hydrogen peroxide for 1 minute?
3. Add on of the following volumes of distilled water to the test tube, as assigned by your teacher: 10.0mL, 15.0mL, 20.0mL, 25.0mL, 30.0mL. (If you use a graduated cylinder, remember to read the volume from the bottom of the water meniscus. You can make more a more accurate volume measurement using either a pipette or a burette.)
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Record any observations made. (A clear positive reaction for the 2% glucose control and a clear negative reaction for the water control should be seen).
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells Introduction Enzymes are made of proteins and they speed up reactions, this means that they act as catalysts. Hydrogen peroxide is a byproduct of our cell's activities and is very toxic. The enzymes in our bodies break down the hydrogen peroxide at certain temperatures they work best at body temperature, which is approximately 37 degrees. At high temperatures, the cells begin to denature. This means that the hydrogen peroxide is prevented from being broken down because they will not 'fit' into the enzyme.[IMAGE] Objective I am going to find out how the concentration of the substrate, hydrogen peroxide affects the reaction in the catalase inside the potato cells.
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
2. In the large beaker, put water and boil it completely. After that, remove the beaker from heat. 3. Sample tubes (A-D) should be labeled and capped tightly.
The Concentration of Hydrogen Peroxide and Speed of The Rate at Which It is Broken Down by Catalase