5. Problems:
1. In which pH will the enzyme catalase in the potato sample catalyze hydrogen peroxide most effectively?
2. Will the amount of hydrogen peroxide in relation to the amount of potato sample affect my results?
3. Will crushing the potato sample lead to different results, than if a whole potato is used?
4. What is the optimum pH for the enzyme catalase?
5. Will the enzyme catalase actually catalyze hydrogen peroxide in an alkaline medium?
6. Hypothesis
I predict that the enzyme activity of catalase on hydrogen peroxide will be more effective in an acidic pH and less effective in an alkaline pH over a period of 10 minutes.
7. Aim
To determine the effects that the different pH levels of water, vinegar and oven cleaner will have on the enzyme catalase while it catalyzes hydrogen peroxide.
8. Variables
8.1 Dependent variables
The time taken for the enzyme catalase to catalyze hydrogen peroxide (until there are
…show more content…
My hypothesis was incorrect, as catalase did not catalyze hydrogen peroxide the most effectively in an acidic pH. In the experiment we saw that catalase in the alkaline solution catalyzes hydrogen peroxide the least effectively, because it had almost no bubbles, which means that the hydrogen peroxide was not broken down into water and oxygen. The catalase in the acidic solution catalyzes a small amount of the hydrogen peroxide as there were a few bubbles, which means that the hydrogen peroxide was not completely broken down into oxygen and water. Therefore it was not very effective. The catalase in the neutral solution catalyzes hydrogen peroxide the most effectively, because it produced the most bubbles and bubbled for the longest period of time, which means that the hydrogen peroxide was completely broken down into water and oxygen. Therefore we can conclude that the enzyme catalase works best in a neutral pH and least effectively in an acidic and alkaline
After conducting this experiment and collecting the data I would have to say that the optimal temperature for enzyme activity would have to be room temperature which in my experiment was thirty-four degrees Celsius. I came to this answer because the glucose test strip showed that at room temperature there was more glucose concentration that at either of the other temperatures. Due to temperature extremes in the boiling water the enzymes could no longer function because the breakdown of lactose stopped. The cold water also hindered the breakdown of the lactose but as the water warmed the enzymes were more active which can be seen in the results for the cold water at 20 minutes B. Describe the relationship between pH and the enzymatic activity of lactase.
The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
Is there a difference in the rate of reaction of catalase activity between pinto beans and carrots? Based on our research, we believe that the catalase activity in pinto beans will increase more relative to the catalase activity in whole carrots because pinto beans are higher in protein. We conducted an experiment to test our hypothesis that if we increase the hydrogen peroxide concentration then we will see higher kinetic saturation in pinto beans over whole
The alternate hypothesis is that there exists an optimal pH level for catecholase enzyme in which the catecholase enzyme can operate with the highest possible
With this information we were able to identify any patterns and similarities. Hypothesis: The higher the temperature of water, potato and H²O², the rate at which the Enzyme will work will be faster therefore producing more oxygen. The reaction will be the same without the catalase (potato). Therefore in both experiments the Enzyme will work more rapidly and produce more oxygen. Aim: To test the hypothesis.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Investigating Factors that Affect the Rate of Catalase Action Investigation into the factors which affect the rate of catalase action. Planning Aim: To investigate the affect of concentration of the enzyme catalase on the decomposition reaction of hydrogen peroxide. The enzyme: Catalase is an enzyme found within the cells of many different plants and animals. In this case, it is found in celery.
= I predict that if the concentration is high in the yeast then the speed of oxygen produced in the reaction with hydrogen peroxide will also be high. This is because the amount of yeast that can react with the hydrogen peroxide can get no higher and will have the maximum affect on the reaction. If the concentration is more in favour of water then the amount of oxygen produced will be slow because there is not as much yeast to react with the hydrogen peroxide, giving less oxygen. If the temperature is not in favour of the limits to the yeast then the amount of oxygen produced will be small because the enzyme will have denatured. If the temperature is in favour of the yeast then the amount of oxygen produced will be high because it is at the prime temperature for the yeast to react.
Materials used in the experiment included 5-7 g of the potato tissue, 50ml of 2.0M phosphate buffer coffee filter and guaiacol dye.
After that, from pH 9 to pH 10.2 we can see that the m.r.r. drastically decreases but still remains higher than all the rest m.r.r before pH 9. Discussion: The results of this experiment prove my hypothesis that the pH of the catalase will alter the function of the enzyme, thus the rate of reaction will change. They also prove my other hypothesis that there is a specific pH level in which the catalase works best. However, after looking at Figure 4 we can see that that optimum pH is 9 which, as mentioned in the background knowledge section, is not what would usually be the optimum pH for catalase when conducting this experiment but would be around pH 7.
The effect of a change in PH on enzymes is the alteration in the ionic
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
The first experiments investigate the order of reaction with respect to the reactants; hydrogen peroxide, potassium iodide and sulphuric acid by varying the concentrations and plotting them against 1/time. An initial rate technique is used in this experiment so ‘the rate of reaction is inversely proportional to time.’ To find the order of reaction in respect to the reactants, 1/time is plotted against the concentration of Hydrogen Peroxide using the equation:
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells