Abstract
Background: The oral cavity harbours a large number of bacterial species as normal flora existing as biofilm. Dental disease such as dental caries results when there is a shift in the balance of bacteria towards pathogenic species within these biofilms.
Objective: The objective of this study was to isolation, identification and characterisation of oral bacterial species of patients with dental caries and caries-free healthy control subjects.
Materials and Methods: A standard bacteriological procedures were followed in the isolation of bacteria. The identification of bacteria was carried out using matrix-associated laser desorption ionisation–time of flight–mass spectrometry (MALDI–TOF–MS) (Bruker MALDI Biotyper system). The characterisation
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Dental caries is very common and up to 80% of the world population may suffer from some form of this disease during their lifetime15,16. Culture-independent metagenomic studies revealed that more than 6 billion bacteria are present in the oral cavity of human representing more than 700 bacterial species which contribute to the health and physiological status of the oral cavity; only a fraction of which can be cultivated in conventional laboratory …show more content…
Isolation of this bacterium from a healthy subject highlights the fact that, although the bacterium exists as a normal flora, it may turn into an opportunistic pathogen and cause serious complications.
In summary, in this preliminary study, we analysed 35 oral samples (10 from patients with dental caries and 25 caries-free subjects) and 17 samples yielded identifiable bacterial colonies. A total of 13 different bacterial species were isolated from these 17 samples. Out of these 13 different bacterial species, an extensive survey of scientific literature showed that seven are isolated for the first time in Saudi Arabia.
CONCLUSION
In conclusion, we reported the isolation of seven oral bacterial species (4 of which are Streptococcus spp.) for the first time from Saudi population. However, the biological significance of these oral bacterial species was not the focus of this study, and it remains to be evaluated in large-scale controlled
The purpose of this study is to identify an unknown bacterium from a mixed culture, by conducting different biochemical tests. Bacteria are an integral part of our ecosystem. They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species. These biochemical tests are process of elimination that relies on the bacteria’s ability to breakdown certain kinds of food sources, their respiratory abilities and other biochemical conditions found in nature.
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
The purpose of this project was to identify unknown bacteria species from a mixed culture. The two unknown species were initially plated onto Tryptic Soy Agar (TSA), Eosin Methylene Blue (EMB), Mannitol Salt Agar (MSA), and blood agar plates to distinguish between the two different bacteria using colony size, color, shape, and growth characteristics. By identifying and inoculating the differing types of colonies, the two unknown bacteria were purified and able to be tested
The article Poor Teeth was written by Sarah Smarsh with the goal in mind being to shed light on the issue between upper and lower class society in a particularly concrete way. Teeth and dental health are an easy thing for people to imagine in their head because everyone has a set whether they’re white and shiny or black and rotted. This makes it easy to draw a comparison between people that care for their teeth and those who don’t. However, access to dental knowledge and services which the lower class often times doesn’t have is very different between the poor and the rich. While the rich stroll through life showing off their perfect glossy white rows of teeth, there are less privileged people out there with barren mouths whose weak pale gums
Candida albicans is a harmless commensal yeast which becomes pathogenic when environmental changes trigger the virulence factors of the organism. Hence Candida species are opportunistic pathogens in susceptible individuals. Oral infection caused by C. albicans known as oral candidiasis or candidosis. However, C. albicans exist in the mouths of 80% of healthy individuals. Any alteration in the environment of oral cavity can change the presence of candida from commensal to pathogenic. This translation in the status of candida is due to many predisposing factors. In the past, oral candidiasis was thought to affect mainly elderly and very young population. Recently, the incidence of oral candidiasis increased greatly with the intensification of HIV infection and immunosuppressive chemotherapy (1, 2). Oral candidiasis colonize 5% to 7% of newborn less than one month old. The infection also estimated to affect 9% to 31% of AIDS patients and nearly 20% of cancer patients (3). This review discuss the possible causes, the types of oral candidiasis, treatment and management strategies.
Based on aim 1 of the experiment, the goal is to find a connection between braces and canker sores by testing the microbiota. For this test, participants will have the oral microbiota profiled based on those with braces and those that do not have braces, with a special focus on canker sore formation. Each of the participants will have an oral examination completed before the experiment is performed and again after its conclusion, to detect any differences that could occur with canker sores. This test will se...
This topic was brought to my attention by noticing the lack of dental hygiene among my peers. This doesn’t necessarily just mean having cavities, sore gums, or bad breath. No one likes communicating with another person with bad, stinky breath. Bad breath is also known as halitosis, which is caused by anaerobic bacteria. Anaerobic bacteria is found in our mouth from drinking coffee, eating spicy foods,smoking tobacco, medications, gum diseases and many more.
o Please describe a time when you performed a thorough head, neck and oral exam and the findings had a significant impact on how you proceeded with the dental hygiene process of care. Annette was performing her usual head, neck, and oral exam on a patient and found an enlarged thyroid. She recommended to the patient that she go see her primary care physician to get a better diagnosis. The patient went to her primary care physician and was told there was probably nothing. Later, the patient returned to the dental office and saw Annette and told her what the physician said.
Streptococcus pyogenes is thought to live benignly within one in five people, and is thusly one of the most common pathogens among humans. Due to its common
Diabetes mellitus affects an estimated 20 million Americans, about 35 to 40 % of whom have not received a diagnosis. More than 9 % of the adult population has diabetes, and both the incidence and prevalence are increasing every year.” (Mealey, 2006, page 1) This is a scary statement. These numbers include our parents, aunts, uncles, sisters and brothers. We need to be aware that this is a real threat. Many of these individuals most likely have some type of periodontal issues as well, particularly if the diabetes is uncontrolled or not yet diagnosed. The health of the mouth can play a significant role in the overall health of the body.
Due to their direct contact with patients, personnel of health care teams are exposed to a higher risk of contracting diseases. Specifically, this risk becomes more potential for dental professional teams if we know that most of the human microbial pathogens are isolated from oral secretions. They can cause serious diseases that might transmit from patients to the personnel, and vice versa (Bednarsh & Molinri, 2010). In 1970 and the early 1980s, result showed the tendency among dental professionals to have more certain infectious diseases than the general public due to their exposure to blood and saliva of their patients (Bednarsh & Molinri, 2010). Disease, such as hepatiti...
Dental professionals should promote a message that a strong biologic connection exists between oral and systemic health. Therefore, having a good understanding of oral health and systemic health is beneficial and can help you protect yourself. Streptococcus mutants, the bacteria found in periodontal infection are also the bacteria found in many of the cardiovascular diseases. This establishes a strong relationship between these two conditions.
However, on the a recent visit dated 23/12/13 the patient’s gingival condition had deteriorated, presenting with an increased plaque scores of 34% and bleeding scores to 63%. Intra oral examination also showed generalised oedema and erythema throughout in the mouth in response to this increase in plaque bacteria. The presence of supra-gingival calculus on lower anterior teeth and both sites of upper buccal molars and the patients BPE now reads 212 /121, putting the patients caries risk at a ‘High’ status.
Dental plaque is the combination of bacteria embedded in a matrix of salivary proteins and bacterial products superimposed on the acquired pellicle (Berkovitz, et al., 2011). It is an example of a biofilm, a term used to describe relatively undefinable microbial community associated with a tooth surface or any other hard, non-shedding material (Reddy, 2008). Plaque can also be described as
Saliva contains glucose; therefore, if you have uncontrolled diabetes, the amount of glucose present in your saliva is elevated. We naturally have somewhere between 200 to 300 bacterial species in our mouths: Some of these bacteria are good and some of them are bad. Streptococcus mutans is typically the bacterium responsible for causing tooth decay; whereas, periodontal disease is usually caused by a mixture of Porphyromonas gingivalis and Treponema denticola.