BMI-1 Autoantibody as a New Potential Biomarker for Cervical Carcinoma. The purpose of this study was to determine whether there was any correlation between elevated levels of the BMI-1 autoantibody and cervical carcinoma. In order to determine whether any correlation existed, the levels of the autoantibody BMI-1 in patients who had cervical carcinoma of varying stages and those who did not have cervical carcinoma were compared. Overexpression of BMI-1 has already been associated with certain cancers such as leukemia amongst others; this study was specifically looking at cervical carcinoma as a possible cause for the overexpression of BMI-1. This study utilized HeLa, Caski, and SiHa lines of cervical carcinoma cells as well as H8 line …show more content…
Peripheral blood samples were collected from 150 individuals for use in this study. 77 of the 150 samples were collected from individuals with histologically confirmed cervical carcinoma. 25 of the 77 samples were collected from individuals with stage I cervical carcinoma. 23 of the 77 samples were collected from individuals with stage II cervical carcinoma. 18 of the 77 samples were collected from individuals with stage III cervical carcinoma. 11 of the 77 samples were collected from individuals with stage IV cervical carcinoma. 73 of the 150 samples were collected from healthy individuals with no cervical carcinoma. Ten cervical carcinoma sera samples and eight normal sera samples were used to biopan; the remaining 67 cervical carcinoma samples and 65 normal samples were used to evaluate predictive value by receiver operating characteristics (ROC) curves. Total RNA was extracted from the sample cells using the acid guanidinium thiocyanate-phenol-chloroform method using Trizol as the reagent. Random hexamers were used in cDNA synthesis. BMI-1 was amplified by polymerase chain reactions (PCR). The PCR products were analyzed using agarose gel electrophoresis to confirm appropriate size and sequencing. Cells were washed and
Del Puerto, H. L., Martins, A., Vasconcelos, A. C., Milsted, A., Souza-Fagundes, E. M., Braz, G. F., et al. (n.d.). Canine distemper virus induces apoptosis in cervical tumor derived cell lines. http://www.virologyj.com/content/8/1/334. Retrieved January 29, 2014, from http://eds.b.ebscohost.com/eds/pdfviewer/pdfviewer?sid=c0b3ec67-82b8-411a-a30b-
Background and objective. Tumor heterogeneity is shown to be related to clinical outcome in cancer patients. The concept of a small subset of cancer stem cells being responsible for tumor relapse and metastasis comes out as a promising strategy for targeted cancer therapy. However, cancer stem cells are not easy to identify and isolate. The aim of this study was to determine the putative colon cancer stem cell subsets in human colon cancer cell lines HCT116 and HT29, which differ in their aggressiveness and differentiation capacity. Material and methods. Flow cytometry was used to asses HCT116 and HT29 cell lines for the expression of stemness-associated surface markers CD24, CD44, CD117, CD133, ESA, ABCB1. Both cell lines were treated with 5-fluoruracil and the phenotype of chemoresistant cells was investigated. Side population was visualized via Rhodamine 123 staining. Relative expression of ABCG2, c-Myc and Oct4 genes was quantified using qPCR analysis. Results and conclusions. It was shown that HCT116 and HT29 cell lines differ in their stemness-related properties. We imply that putative CSC subset for HCT116 cell line is CD44+/CD24-/CD133- (4,1% of all cells) and for HT29 cells – CD24+/CD44-/CD133- (4,9% of all cells).
You will be evaluated on completeness, thoroughness, and accuracy. The goal of this project is for you to accurately determine and professionally interpret the results as if you were doing so for a patient. Assume that the person you are answering these questions for has never heard of body composition or what it means. Answers must be complete.
Although, it is easy to believe that all cells in a tumor are neoplastic, evidence suggests otherwise. There are three characteristics that are present in all KS cells whether they are neoplastic or not. The first is absence of a histologically distinguishable neoplastic cell. The second is the lack of usual chromosomal abnormalities. The last is a combination of three features angiogenesis, inflammation, and proliferation.
The overall aim of this quality improvement is to provide an improved assessment for defining if individuals are truly overweight or obese though utilising BMI measurements alongside BIA measurements. For those individuals who are border lining the outlines of the BMI cut off for assisted reproduction ranges from 29.1 to 30 kg/m2, to identify if the individual is carrying excess body fat or if the individual carry’s extra lean mass or excess bone density. Which in some cases these individuals could be refused treatment due to the assumptions
Adams, Heather P., and Erica L. Carnright. "HPV Infection And Cervical Cancer Prevention." Clinician Reviews 23.9 (2013): 42-50. Academic Search Complete. Web. 10 Nov. 2013
INTRODUCTION: Apoptosis is a distinct form of a programmed cell death ( PCD) and defect in apoptosis are now thought to contribute to the development and progression of cancer. Regarding the keen interest in programmed cell death in the last years, more and more assays and commercial kits are emerging to prove apoptosis. However, their detectability and reliability have been often discussed. Here we introduce rapid and simple method for evaluating apoptosis in cancer researches and genotoxicities. METHODS: NIH-3T3 cell line were used in this study, after treatment with apoptotic agent, DNA was extracted by simple protocol. Then DNA ladder and flow cytometry assays were done for detection of apoptosis in NIH-3T3 cell line. RESULT: Primary and late apoptosis in the treated cells was determined by flow cytometry analysis. Accordingly DNA ladder assay using 1.5 % gel electrophoresis showed fragmentation in the DNA of treated cells. CONCLUSSION: This research provides a fast, simple and cost-effective method in observing apoptosis in mammalian cells and could be use in cancer research and genotoxicity.
Cervical cancer is formed in the tissues of the cervix, an organ that connects the uterus and the vagina. Virtually all cervical cancers are caused by Human papillomavirus (HPV) infections (Schiffman et. al., 2007). HPV is the most common sexually transmitted infection in the United States. According to the CDC, 75% of sexually active people aged 15-49 have the infection at some point in their lives. (CDC). Because HPV infection is usually asymptomatic, infected people do not know exactly when they get the infection. In most cases, the body is able to fight off the virus before any symptom. However, health problems such as genital warts and cancer may result with persistent exposure to HPV.
Penberthy, L., Gillam, C., Ginde, G., Mcclish, D., Peace, S., Gray, L., . . . Radhakrishnan, S. (2012). Hematologic malignancies: An opportunity to fill a gap in cancer surveillance. Cancer Causes and Control, 23(8), 1253-1264. doi:10.1007/s10552-012-0003-1
Over the years, the fight against ovarian cancer has proven to be even more difficult due to the cancer being asymptomatic at its early stages. For this reason, there are constantly late diagnoses made on women who unfortunately develop this cancer (Stack).... ... middle of paper ... ...
The first component of the MUST involves measuring the patient’s height and weight to establish their Body Mass Index (BMI). BMI is the’ relationship b...
As an inducer of HIF-1 production, it’s been used to study the apoptotic effects in HepG2 cells.
Uterine cancer is an important women health problem developing rapidly, killing over 200,000 women each year. No one has discovered the actual cause, but there is a leading factor that has great suspicions to what is causing this cancer to grow rapidly.
Blood and urine based biomarkers used in molecular pathology are only indicative of the average response of the cell population affected with little or no information of the range of response or variability form areas of tissue (Naddler and Langley 2001)