Antioxidant Activity of Wheat Grains

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The present study was designed to evaluate the antioxidant activity of 5 organic solvent extracts (petroleum ether, n-hexane, chloroform, ethyl acetate and methanol) of wheat grains, 3days, 5 days and 7days old wheat seedlings. To determine the antioxidant activity of five extracts of four different samples, DPPH and ABTS radical scavenging activity, total phenolic content and ferrous reducing power ability were carried out. DPPH radical scavenging effect of chloroform and ethyl acetate extracts of 3d WS was higher than wheat grains. Chloroform, ethyl acetate and methanol extracts of 3d old wheat seedlings exhibited higher ABTS radical scavenging effcet than extracts of other samples. The phenolic content was high in chloroform, ethyl acetate and methanol extract of 5d WS. When compared with WG, reducing power ability was high in chloroform, ethyl acetate and methanol extract of wheat seedlings, especially in 3d and 5d old WS. From the above results, it was concluded that chloroform, ethyl acetate and methanol extract of 3days, 5days and 7days old wheat seedlings showed better antioxidant activity than the wheat grain extracts. Hence, results of the present study suggest the intake of wheat seedlings as a food supplement to combat the diseases caused by free radicals. Key words: Wheat grains, DPPH, ABTS, phenolic content, reducing power ability. Introduction In the present era, people are affected by various diseases due to changes in culture and lifestyle. The changes in food habits and working pattern causes stress which leads to biochemical changes. Research findings relate oxidative stress to a number of diseases like cancer, diabetes, Alzheimer’s disease, coronary heart diseases, and aging[1, 2]. Scientific reports emphasiz... ... middle of paper ... ... also having pronounced effect on the difference in the antioxidant properties of wheat [29-31]. Increase in antioxidant content of 3 to 5 days wheat sprouts was due to germination [32] and the content of fatty acids like palmitic acid, linoleic acid, and oleic acid were also found to be high in wheat sprouts [33]. Previous findings reported that aqueous and ethanol extracts of wheat grass have potent antioxidant activity and inhibit proliferation of leukemia cells [34] and the antioxidant properties were changed by lighting effects [35]. The objective of the present study was to evaluate the antioxidant activities of five different extracts (petroleum ether, n-hexane, chloroform, ethyl acetate, and methanol) of wheat grains (WG), 3days (3d), 5days (5d) and 7days (7d) old wheat seedlings (WS) using DPPH, ABTS, Phenolic content and Ferrous reducing power ability.

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