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However, at 3% substrate concentration, the hydrogen peroxide decomposition showed an immediate peak of up to 3.8 mm in height. As the substrate concentration slowly increased, enzyme
The process by which high fructose corn syrup is made is complicated. To start, ordinary corn syrup must be obtained. Then, enzymatic processes increase its original sweetness. To produce the basic un-enhanced corn syrup, wet milling is a commonly used technique. Wet-milling includ...
With this information we were able to identify any patterns and similarities. Hypothesis: The higher the temperature of water, potato and H²O², the rate at which the Enzyme will work will be faster therefore producing more oxygen. The reaction will be the same without the catalase (potato). Therefore in both experiments the Enzyme will work more rapidly and produce more oxygen. Aim: To test the hypothesis.
Cellulosic ethanol will not be able to meet its mandated output because in the past four years since demonstration and pilot sites started popping up it has only just started t...
Cellulases are o- glycosyl hydrolases (GHs) that hydrolyse β-1,4 glucosidic bonds in cellulose. Cellulase system is grouped into “glycoside hydrolases (GH) family” classified by different means, according to their substrate specifities, reaction, mechanisms or structural similarities. The cellulase complex is found to contain three basic components which may be present either as single polypeptide or can be grouped together into multienzyme complex known as cellulosome. Cellulase system is composed of three main classes based on their activity toward a wide range of substrates. This is rather difficult, since the enzymes have overlapping specificities toward substrates which themselves are poorly defined. The three main classes are:
Fermentation is a form of chemical transformation of organic substances that breaks down simple compounds by exploiting the enzymes with compl...
Michael P. Broadribb, C. (2006). Institution of Chemical Engineers . Retrieved July 26, 2010, from IChemE: http://cms.icheme.org/mainwebsite/resources/document/lpb192pg003.pdf
Despite of general properties of enzymes, the properties also varies from where it comes from and how it been produced. For instance, the enzymatic saccharification method in lignocellulosic bioethanol is generated by hydrolyzing cellulose and hemicelluloses. This method gets high attention because of its higher theoretical yield compared to other methods (Taneda et al., 2012). Acremonium cellulolyticus with high activities of cellulase, amylase and pectinase enzymes allow it for the easy separation of solids/liquids in potato pulp, resulting in high saccharification efficiency and a high recovery rate of products (Gao et al., 2014). On the other hand, Enzyme-modified carboxymethyl starch (ECMS) is beneficial in enhancing water holding capacity, emulsion stability and improving sensory characteristics of sausages with a declined fat content (Luo and Xu, 2011). Lipases and phospholipases of dormant cotton seeds have stability in heat, various media and nature of the hydrolysis of the lipids properties (Rakhi...
Cushman, Lynd, Nichols, Wyman. “Fuel Ethanol from Cellulosic Biomass.” Science. March 1991. Vol 251 (4999):1321
The Effect of Surface Area on the Rate of Reaction Between Catalase from a Potato and Hydrogen Peroxide
Influence of Temperature on the Activity of Potato Catalase Hypothesis That the higher the temperature the higher the reaction rate of potato catalyse to a point were denaturing occurs in the enzyme and the reaction rate of the potato catalase drops off. Prediction The rate of Catalase activity will be faster at higher temperatures until a point, because at higher temperatures there are more chances of collisions between the enzyme's (Catalase) active site and the substrate (hydrogen peroxide). However the rate depends on the active site being able to join with the substrate, and at higher temperatures the enzyme can be denatured, which changes the shape of the active site which thus prevents the reaction from happening. At first, as the temperature increases the activity of the Potato catalase also increases this is because the collision rate of the enzyme with the hydrogen peroxide is increased.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
By taking a Carbon Dioxide, rich substance and mixing it with a yeast, solution fermentation will occur, and then it could be determined if it is a good energy-producer. In this study glacatose, sucrose, glycine, glucose, and water were used to indicate how fast fermentation occurred. The overall result shows that monosaccharides in particular galactose and glucose were the best energy source for a cell.
Biomass gasification is a process by which biofuel is produced. It has been used for over 180 years but in the last decades it has been reconsidered as an interesting technique due to the fact that oil supplies are decreasing. As mentioned before, gasification is a thermal process. Heat is added up in order to convert the organic mass to biofuel. The biomass usually undergoes drying, pyrolysis, partial oxidation and reduction. Nowadays the configurations used for gasification are three: fixed bed gasifier, fluidized bed gasifier and entrained bed gasifiers. The simplest configuration is the
The process of alcoholic fermentation begins with the use of enzymes. The enzymes begin to break down the long chains in starch molecules, a polysaccharide that consists of a large quantity of glucose molecules (C6H12O6) joined by glycosidic bonds as seen in figure 1, into single glucose molecules, a monosaccharide with six carbons and five hydroxyl groups. After the starch has become sugar, the enzymes are used once again, this time to convert the sugars into ethyl alcohol and carbon dioxide, CO2, as seen in figure 2 (World of Scientific Discovery, 2007). The carbon dioxide produced is released into the atmosphere, leaving water and ethanol, the alcohol, behind. Ethanol is a colorless flammable liquid with a molecular formula of C2H6O, giving it a molar mass of 46.07 grams per mole. Ethanol is also characterized by a melting point of -114°C or 159 K.