preview

Analysis Of M. Zapota Seed Extract

Good Essays
Preparation of M. zapota seed extract:
The seeds of M. zapota were collected freshly from the farms in Chennai, Tamil Nadu. The seeds were rinsed well with distilled water and air dried for two weeks before grinding them into a fine powder. The crude extract was prepared by dissolving 1gm of the powdered seeds in 10 ml of hexane, acetone and ethanol respectively under continuous shaking condition for 24 hours.The process is repeated thrice using fresh solvents. The solvent was removed by condensation and extracted residues were weighed in different solvents to yield 10mg/ml stock solutions[12].
Screening of phytochemicals:
The following tests were performed on the extracts to detect various phytoconstituents present in them with reference to
…show more content…
0.5ml of extract along with 0.1 ml of (0.5N) Folin-Ciocalteu’s reagent was incubated at room temperature for 15 min. After incubation, 2.5 ml of saturated sodium carbonate solution was added and further incubated for 30 min at room temperature. The absorbance was measured at 760 nm. Gallic acid was used as a positive control. Total phenol content was expressed in terms of gallic acid equivalent (mg/g of extracted compounds).The assay was carried out in triplicates and expressed as mean±SD.
Determination of flavonoids
The amount of total flavonoids in the acetone extract of seeds of M.zapota was determined by aluminium chloride colorimetric method [16]. The reaction mixture 3 ml consisting of 1 ml of sample (1 mg/ml), 0.5 ml of (1.2%) aluminium chloride and 0.5 ml (120mM) potassium acetate was incubated at room temperature for 30 min. The absorbance of the reaction mixture was measured at 415 nm. Quercetin was used as positive control. The flavonoid content was expressed in terms of quercetin equivalent (mg/g of extracted compound). The assay was carried out in triplicates and expressed as mean±SD.
Determination of
…show more content…
Briefly, 0.1 ml of the sample extract was added to 7.5 ml of distilled water, 0.5 ml of Folin Phenol reagent, 1 ml of 35% sodium carbonate solution and was diluted to 10 ml with distilled water. The mixture was shaken well, kept at room temperature for 30 min and absorbance was measured at 725 nm. Gallic acid was used as a positive control. Values were expressed in terms of gallic acid equivalent (mg/g of extracted compounds).The assay was carried out in triplicates and expressed as mean±SD.
Thin layer chromatography and Bioautography:
The seed extract with a concentration of 1mg/ml was spotted on the TLC plates.The solvent system used was acetone and hexane with various ratios. The spots were identified in long UV, short UV and also in the iodine chamber. Then RF value was calculated to find the active metabolites. The developed TLC plates were sprayed with DPPH to study the zone of inhibition. The compounds having antioxidant property will exhibit a clear zone over the plates [18].
Profiling of secondary metabolites by Gas chromatography-Mass spectrometry
Get Access