Aloe gel is the clear, jelly-like substance found in the inner part of the aloe plant leaf. Aloe latex comes from just under the plant's skin and is yellow in color. Aloe vera contains 99.3 percent water and 0.7 percent nonaqueous constituents, such as glycoproteins and polysaccharides etc. Glycoproteins speed the healing process by stopping pain and inflammation1, while polysaccharides stimulate skin growth and repair5,6. These substances may also stimulate the immune system.
It is said to be a natural cleaner, powerful in penetrating tissues, relieving pain associated with joints and muscles, bactericidal, a strong antibiotic, virucidal when in direct contact with long periods, fungicidal, anti-inflammatory4, instrumental…show more content… 1.2…show more content… The leaf was cut into cubical pieces (chunks). The upper and lower skin of each leaf was then carefully removed with the help of knife and the mucilaginous mass made up of parenchymatous tissue (filets) was cut into small pieces. Care was taken to avoid contamination of yellow sap with the filet. Filets were inspected visually for any contamination or adhering yellow sap or any outer green skin and discarded if necessary. The total mass of the uncontaminated fillet was approximately about 40% of the leaf mass.
The uncontaminated filets were homogenized twice in a blender at a high speed for 5 minutes. The foam was developed which was settled after 10 minutes. The extracted gel was subjected to straining through muslin cloth to remove traces of cellular matter. The clear gel was then subjected to further processing.
Preparation of mucoadhesive film:
1. Weighed quantity of Aloe Vera juice was taken and kept at room temperature for 10-15min (since juice was taken from refrigerator).
2. HPMC was dissolved in 10ml of distilled water with gentle stirring.
3. Acrylate copolymer solution was prepared in ethanol into another beaker.
4. To HPMC dispersion, Eudragit RLPO solution was added and then stabilized Aloe Vera juice with respective conc. was added along with PEG 400 was added and stirred