An Experiment on the Use of Immobilised Glucose Isomerase
Apparatus:
1) 15g of Fructose
2) 1g of Immobilised Glucose Isomerase
3) 8 Clinistixs’
4) 250 ml beaker
5) Kettle
6) Thermometer
7) 8 test – tubes’
8) Stirring rod
9) 1 pipette
10)2 syringes
Method:
Firstly 10 cm ³ of fructose solution was placed into a test tube with
a syringe. Then a clinistix was dipped into the solution for 10
seconds which it was then removed and placed against the standard
strip where the colour would be observed. Then 1 gram of the
immobilised glucose isomerase was added to the test tube and was
incubated in at 65 °c, with occasional stirring. Then after 5 minutes
a small sample was removed with a pipette after allowing the liquid to
cool before testing with a clinistix. And the colour was recorded for
the results. This test was repeated at every 5 minutes intervals until
a maximum colour had been obtained. Then the immobilised enzyme was
left to settle out and then the liquid was pipette off. Then 10cm of
distilled water was added to the same test tube, where the test tube
was gently mixed with a stirring rod, where then the water was
removed. Then a further 10cm of fructose solution was added to the
test tube and a test was taken immediately. Then again the test tube
was placed into a water bath of 65 °c, where here the test was
repeated two times, where the results where recorded.
Results:
Time
Clinistix Colour
Is the colour Negative/Positive
0
Pink
Negative
5
Dark Purple
Positive
10
Navy Blue
Positive
0
Pink
Negative
5
Dark Purple
Positive
10
Navy Blue
Positive
0
Pink
Negative
5