Amylase Essay

Then transferred each beet to a separate test tube containing deionized water. After 20 minutes in these diffusion solutions, we took the beets out with a dissecting needle and discard it. We then stirred each solution in the test tube with a stirring rod, and transferred it to a cuvette. A spectrophotometer was then calibrated, and used to measure the absorbance of each exposure solution, and diffusion solution. Membrane Damage For the lab experiment for Membrane Damage, we tested the extract pigment and diluted it.

Examine each tube and record the solution color in the table 7. Rinse out the tubes; use the same labels for the iodine test Iodine Test for Starch 1. Set up tubes and label 2. Add 1 mL of each sample to be tested. Make sure you stir the solution before pipetting it into your tube 3.

First, you obtain a sample of saliva and do so by putting a couple of ounces of water in your mouth, swishing it around, and then spitting it into a small cup. Then, you label the two test tubes with your initials on them and labeling them by number one and number two. Then, you add ten drops of starch solution and ten drops of water to the first test tube. For the second test tube you do the same as well. After this is done, you then place both test tubes in the water bath for thirty minutes.

(SDS denatures the proteins, BPB is used as a tracking dye while Beta Mercaptoethanol breaks sulfide bonds.)  Heat the samples at 80 degrees Celsius for 5 minutes in a water bath. This helps in protein denaturation.  Put the cassette into the buffer dam and fill it with the running buffer. For SDS-PAGE, running buffer is prepared by taking 40 ml Tris-Glycine and adding 4 ml of 10% SDS to it.

3.3 Colony PCR Single colonies were selected using sterile 10 μl pipette tip and inoculated with 0.2 PCR tube. Colony PCR was performed with each reactions consists of around 1 μl cell culture, 2.5 pmol of Grb14 forwa... ... middle of paper ... ...rm (1:1) was added to the linearized sample in a 1.5 ml microcentrifuged tube. The mixtures were centrifuged at 13000 rpm for 2 minutes at room temperature. The upper aqueous solution was transferred to another sterile 1.5 ml microcentrifuged tube. Equal volume of chloroform was added and centrifuged at 13000 rpm for 5 minutes.

5ml of starch was put into a test tube. The test tube was placed into the beaker. When the water in the beaker was at the required temperature the stop clock was started. After one minute 1ml of amylase was put into the test tube with the starch using a syringe or pipette. As soon as the amylase and the starch had mixed a sample was taken from the test tube using a pipette and mixed with the droplets of iodine in one of the chambers of the spotting tile.

Appearance of green, yellow or red solution depending on the amount of reducing sugar present in the test solution which indicated the presence of reducing sugar. Tests for Protein and Amino acids:  Biuret’s Test: The extract was treated with 1 ml of 10% sodium hydroxide solution in a test tube and heated. A drop of 0.7% copper sulphate solution was added to the above mixture. Appearance of violet or pink colour indicated the presence of proteins.  Ninhydrin Test: 3 ml of the test solution was heated with 3 drops of 5% Ninhydrin solution in a water bath for 10 minutes.

This was to see the difference between the initial temperature and the final temperature. First Test In a 250ml beaker place 100mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved. After 1 minute measure the temperature and record it, do this for a further 2 minutes (3 minutes in total). Repeat this process for a total of 10 teaspoons.

Prepare .05 to .075 g of crude lipid by dissolving it in hexane. Add to the silica gel slurry in the column. Begin collecting samples with the pure hexane. Keep adding hexane so that the silica gel column does not run dry. Collect one 20 ml sample.

The measurement was .995g. The salicylic acid was transferred into a 125 ml Erlenmeyer flask and the acetic was given by the lab instructor. Three drops of 85% phosphoric acid. While swirling and mixing more was added and then clamp onto the sing stand in the boiling water for 8 minutes. 1 mL drops of water was added in the flask.