Based off of the gram reaction, the tests I chose to do were the Oxidase, Sulfur reduction, Indole Production, Motility(SIM), Citrate Utilization, Urease and Methyl Red and Voges-Proskauer (MRVP) Test. Microscopic Examination: The cell morphology is an important characteristic that helps identify bacteria. The three main shapes of bacterial cells are coccus, bacillus, and spirillum (3). Stains were used in order to facilitate the viewing of bacterial
coliform as well as Escherichia coli. Humans as well as animals come into contact with these areas, some are used for recreational activities such as swimming and some are a source of drinking water for both animals and humans The main goal of this experiment was to see which lakes, snow run off and ponds tested positive for coliform or Escherichia coli and to come up with some reasoning as to why. It was found that the more remote pond with less contact contained the most Escherichia coli. However, another
factors tend to arise during a comparison of them. One of the most notable areas that viral, fungal and bacterial meningitis differ in are their treatment ability. However, they have the same general affects on the human body. In any case, there are tests that doctors can utilize in order to discover if the meningitis is bacterial, fungal, or viral. Meningitis is a contagious infection of the cerebrospinal fluid and inflammation of the meninges, the nearby membrane that covers the spinal cord and brain
virus, Protista and bacteria. It’s important to know and to identify what kind of bacteria and how we can treat it since is found everywhere in society. Also to know what kind of bacteria it is by the performing different biochemical test and be able to differentiate the bacteria. This is use in the medial field where is important to know what kind of bacteria there dealing with and know how to treat it. The purpose of the study was to identify what are unknown bacteria by applying all the methods that
antimicrobial use practices for the sampled group of animals beginning in the farrowing phase. Fecal samples were shipped overnight to The Ohio State University for bacterial culture of E. coli and Salmonella, and selective enrichment with media containing cephalosporins: E.coli: For blaCMY-2 selective E. coli culture, a 4 gram aliquot of feces was added to 36 ml of nutrient broth containing 4 µg/ml cefoxitin and incubated at 37° C for 24 hours. The samples were then streaked onto MacConkey agar
acquired either as health care associated or in the community. The cause of such infection also includes the following but not limited to poor hygiene, sex, instrumentation, anatomic structure, etc. [6]and Out of the several causative agents, Escherichia coli and other coliforms played as major causative agents[7,10]. Urinary tract Infections are commonly treated with sulfamethoxazole - trimethoprim and fluoroquinolones[7,10,12]. However, due to the frequency of antibiotic use, recurrent or chronic
INTRODUCTION The ability to identify or know the steps to identify an unknown microorganism requires an intellectual and physical discipline. It also reflects the ability of one’s accumulation of knowledge and skills when able to proceed with identifying unknown bacteria. In the microbiology class, students were given a tube of mixed cultures that needed to be separated to proceed with the identification of just one of the cultures. Through the course of five weeks, materials and methods took
to DNA replication. DNA polymerase, more specifically, is involved in the process of reading and adding nucleotides to the DNA strand so a complimentary stand can be made. During the DNA replication process DNA polymerase puts new nucleotides on the 3’ end of the DNA Strand. Not only does DNA polymerase add nucleotides to a DNA strand it can also act somewhat as a “proof reader”. It can pause the replication process to fix mistakes that can occur during DNA replication. Once the sequencing mistake
Lipps 2003, Lipps 2008 B). Our solution is to create a universal antivenom is modify a strain of Escherichia coli to produce LT-15 (Lipps & Lipps 2005). We will insert the LT-15 gene into a plasmid with a promoter, then transform E. coli with the plasmid and grow the recombinant strain. (Cawood 2013, Cohen et al. 1973, Huang et al. 2012, Lipps 2002 B, Lodish et al. 2000, Muyrersa et al. 2001). These E. coli can then be grown and harvested industrially for LT-15. This is a novel project because this would
denaturation and hybridization of the fragments with a labelled DNA or RNA probe, washing and detection of the hybrids by colorimetric or fluorescence (Chang and Chan 3; Brown 2). Various restriction enzymes can be used to cut the DNA at particular restriction sites leading to the release of DNA fragments with different sizes (Chang and Chan 3). In the Southern blot method, the digested and electrophoresed fragments are transferred and immobilized onto a membrane in such a way that the DNA profile displayed